BMRB Entry 17820

Name: Backbone resonance assignments for IsdH-N2N3
Published: 2011-12-12

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17820

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Backbone resonance assignments for IsdH-N2N3 PubMed: 22101872

Deposition date: 2011-07-29 Original release date: 2011-12-12

Authors: Spirig, Thomas; Clubb, Robert

Citation: Spirig, Thomas; Clubb, Robert. "Backbone 1H, 13C and 15N resonance assignments of the 39 kDa staphylococcal hemoglobin receptor IsdH." Biomol. NMR Assignments 6, 169-172 (2012).

Assembly members:
IsdH-N2N3, polymer, 336 residues, Formula weight is not available

Natural source: Common Name: Staphylococcus aureus Taxonomy ID: 1280 Superkingdom: Bacteria Kingdom: not available Genus/species: Staphylococcus aureus

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
IsdH-N2N3: SADESLQDAIKNPAIIDKEH TADNWRPIDFQMKNDKGERQ FYHYASTVEPATVIFTKTGP IIELGLKTASTWKKFEVYEG DKKLPVELVSYDSDKDYAYI RFPVSNGTREVKIVSSIEYG ENIHEDYDYTLMVFAQPITN NPDDYVDEETYNLQKLLAPY HKAKTLERQVYELEKLQEKL PEKYKAEYKKKLDQTRVELA DQVKSAVTEFENVTPTNDQL TDLQEAHFVVFESEENSESV MDGFVEHPFYTATLNGQKYV VMKTKDDSYWKDLIVEGKRV TTVSKDPKNNSRTLIFPYIP DKAVYNAIVKVVVANIGAEG QYHVRIINQDINTKDD

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	879
15N chemical shifts	292
1H chemical shifts	292

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	IsdH-N2N3	1

Entities:

Entity 1, IsdH-N2N3 336 residues - Formula weight is not available

Residues 1 represents a non-native residue remaining after cleavage of a purification tag.

1

SER

ALA

ASP

GLU

SER

LEU

GLN

ASP

ALA

ILE

2

LYS

ASN

PRO

ALA

ILE

ASP

LYS

GLU

HIS

3

THR

ALA

ASP

ASN

TRP

ARG

PRO

ILE

ASP

PHE

4

GLN

MET

LYS

ASN

ASP

LYS

GLY

GLU

ARG

GLN

5

PHE

TYR

HIS

TYR

ALA

SER

THR

VAL

GLU

PRO

6

ALA

THR

VAL

ILE

PHE

THR

LYS

THR

GLY

PRO

7

ILE

GLU

LEU

GLY

LEU

LYS

THR

ALA

SER

8

THR

TRP

LYS

PHE

GLU

VAL

TYR

GLU

GLY

9

ASP

LYS

LEU

PRO

VAL

GLU

LEU

VAL

SER

10

TYR

ASP

SER

ASP

LYS

ASP

TYR

ALA

TYR

ILE

11

ARG

PHE

PRO

VAL

SER

ASN

GLY

THR

ARG

GLU

12

VAL

LYS

ILE

VAL

SER

ILE

GLU

TYR

GLY

13

GLU

ASN

ILE

HIS

GLU

ASP

TYR

ASP

TYR

THR

14

LEU

MET

VAL

PHE

ALA

GLN

PRO

ILE

THR

ASN

15

ASN

PRO

ASP

TYR

VAL

ASP

GLU

THR

16

TYR

ASN

LEU

GLN

LYS

LEU

ALA

PRO

TYR

17

HIS

LYS

ALA

LYS

THR

LEU

GLU

ARG

GLN

VAL

18

TYR

GLU

LEU

GLU

LYS

LEU

GLN

GLU

LYS

LEU

19

PRO

GLU

LYS

TYR

LYS

ALA

GLU

TYR

LYS

20

LYS

LEU

ASP

GLN

THR

ARG

VAL

GLU

LEU

ALA

21

ASP

GLN

VAL

LYS

SER

ALA

VAL

THR

GLU

PHE

22

GLU

ASN

VAL

THR

PRO

THR

ASN

ASP

GLN

LEU

23

THR

ASP

LEU

GLN

GLU

ALA

HIS

PHE

VAL

24

PHE

GLU

SER

GLU

ASN

SER

GLU

SER

VAL

25

MET

ASP

GLY

PHE

VAL

GLU

HIS

PRO

PHE

TYR

26

THR

ALA

THR

LEU

ASN

GLY

GLN

LYS

TYR

VAL

27

VAL

MET

LYS

THR

LYS

ASP

SER

TYR

TRP

28

LYS

ASP

LEU

ILE

VAL

GLU

GLY

LYS

ARG

VAL

29

THR

VAL

SER

LYS

ASP

PRO

LYS

ASN

30

SER

ARG

THR

LEU

ILE

PHE

PRO

TYR

ILE

PRO

31

ASP

LYS

ALA

VAL

TYR

ASN

ALA

ILE

VAL

LYS

32

VAL

ALA

ASN

ILE

GLY

ALA

GLU

GLY

33

GLN

TYR

HIS

VAL

ARG

ILE

ASN

GLN

ASP

34

ILE

ASN

THR

LYS

ASP

Samples:

sample_1: IsdH-N2N3, [U-100% 13C; U-100% 15N; U-80% 2H], 1.1 mM; potassium phosphate 20 mM; sodium chloride 50 mM; sodium azide 0.01%; D2O, [U-2H], 7%; H2O 93%; Protease Inhibitor Cocktail 0.01%

sample_conditions_1: ionic strength: 70 mM; pH: 6.0; pressure: 1 atm; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CARA, Keller - chemical shift assignment

NMR spectrometers:

Bruker Avance 800 MHz
Bruker Avance 600 MHz

PDB	4IJ2 4XS0
EMBL	CAI81279 COX93366 CPE78318 CPJ07879 CPL95539
GB	AIW27424 EEV26426 EFB57600 EFB60931 EFC00882
REF	WP_042745476 WP_052997956 WP_053014930

Biological Magnetic Resonance Data Bank