BMRB Entry 17908
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17908
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Title: Solution structure Analysis of the ImKTx104 PubMed: 22511981
Deposition date: 2011-09-01 Original release date: 2012-07-23
Authors: Zeng, D.; Jiang, L.
Citation: Chen, Zong-Yun; Zeng, Dan-Yun; Hu, You-Tian; He, Ya-Wen; Pan, Na; Ding, Jiu-Ping; Cao, Zhi-Jian; Liu, Mai-Li; Li, Wen-Xin; Yi, Hong; Jiang, Ling; Wu, Ying-Liang. "Structural and functional diversity of acidic scorpion potassium channel toxins" PLoS One 7, e35154-e35154 (2012).
Assembly members:
entity_1, polymer, 27 residues, Formula weight is not available
Natural source: Common Name: Chinese swimming scorpion Taxonomy ID: 172552 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Lychas mucronatus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity_1: ACVTHEDCTLLCYDTIGTCV
DGKCKCM
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 162 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Acidic Scorpion Potassium Channel Toxins | 1 |
Entities:
Entity 1, Acidic Scorpion Potassium Channel Toxins 27 residues - Formula weight is not available
| 1 | ALA | CYS | VAL | THR | HIS | GLU | ASP | CYS | THR | LEU | ||||
| 2 | LEU | CYS | TYR | ASP | THR | ILE | GLY | THR | CYS | VAL | ||||
| 3 | ASP | GLY | LYS | CYS | LYS | CYS | MET |
Samples:
sample: entity_1, [U-15N], 2 mM; sodium phosphate 20 mM; H2O 90 % v/v; D2O, [U-99% 2H], 10 % v/v
sample_conditions_1: pH: 5; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N-edited 3D NOESY-HSQC | sample | isotropic | sample_conditions_1 |
| 15N,1H-HSQC | sample | isotropic | sample_conditions_1 |
| 1H,1H-TOCSY | sample | isotropic | sample_conditions_1 |
| 1H,1H-NOESY | sample | isotropic | sample_conditions_1 |
Software:
CYANA, Guntert P. - refinement
NMR spectrometers:
- Bruker Avance 800 MHz