BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules 		Member of WWPDB

BMRB Entry 18026

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18026

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Title: Backbone Chemical Shift Assignments for Unmyristoylated GCAP1 bound to Ca2+ ions   PubMed: 22392341

Deposition date: 2011-10-28 Original release date: 2012-03-09

Authors: Lim, Sunghyuk; Ames, James

Citation: Lim, Sunghyuk; Peshenko, Igor; Dizhoor, Alexander; Ames, James. "Backbone (1)H, (13)C, and (15)N resonance assignments of guanylyl cyclase activating protein-1, GCAP1."  Biomol. NMR Assignments 7, 39-42 (2013).

Assembly members:
GCAP1, polymer, 205 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:   Common Name: Cattle   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
GCAP1: MGNIMDGKSVEELSSTECHQ WYKKFMTECPSGQLTLYEFR QFFGLKNLSPWASQYVEQMF ETFDFNKDGYIDFMEYVAAL SLVLKGKVEQKLRWYFKLYD VDGNGCIDRDELLTIIRAIR AINPCSDSTMTAEEFTDTVF SKIDVNGDGELSLEEFMEGV QKDQMLLDTLTRSLDLTRIV RRLQNGEQDEEGASGRETEA AEADG

Data sets:
Data typeCount
13C chemical shifts326
15N chemical shifts131
1H chemical shifts131

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GCAP11
2Calcium ions 12
3Calcium ions 22
4Calcium ions 32

Entities:

Entity 1, GCAP1 205 residues - Formula weight is not available

1   METGLYASNILEMETASPGLYLYSSERVAL
2   GLUGLULEUSERSERTHRGLUCYSHISGLN
3   TRPTYRLYSLYSPHEMETTHRGLUCYSPRO
4   SERGLYGLNLEUTHRLEUTYRGLUPHEARG
5   GLNPHEPHEGLYLEULYSASNLEUSERPRO
6   TRPALASERGLNTYRVALGLUGLNMETPHE
7   GLUTHRPHEASPPHEASNLYSASPGLYTYR
8   ILEASPPHEMETGLUTYRVALALAALALEU
9   SERLEUVALLEULYSGLYLYSVALGLUGLN
10   LYSLEUARGTRPTYRPHELYSLEUTYRASP
11   VALASPGLYASNGLYCYSILEASPARGASP
12   GLULEULEUTHRILEILEARGALAILEARG
13   ALAILEASNPROCYSSERASPSERTHRMET
14   THRALAGLUGLUPHETHRASPTHRVALPHE
15   SERLYSILEASPVALASNGLYASPGLYGLU
16   LEUSERLEUGLUGLUPHEMETGLUGLYVAL
17   GLNLYSASPGLNMETLEULEUASPTHRLEU
18   THRARGSERLEUASPLEUTHRARGILEVAL
19   ARGARGLEUGLNASNGLYGLUGLNASPGLU
20   GLUGLYALASERGLYARGGLUTHRGLUALA
21   ALAGLUALAASPGLY

Entity 2, Calcium ions 1 - Ca - 40.078 Da.

1   CA

Samples:

sample_1: TRIS, [U-100% 2H], 5 mM; DTT, [U-100% 2H], 5 mM; H2O 93%; D2O, [U-100% 2H], 7%; GCAP1, [U-100% 15N], 0.7 mM

sample_2: TRIS, [U-100% 2H], 5 mM; DTT, [U-100% 2H], 5 mM; H2O 93%; D2O, [U-100% 2H], 7%; GCAP1, [U-100% 13C; U-100% 15N], 0.7 mM

sample_3: TRIS, [U-100% 2H], 5 mM; DTT, [U-100% 2H], 5 mM; H2O 93%; D2O, [U-100% 2H], 7%; GCAP1, [U-13C; U-15N; U-2H], 0.7 mM

sample_conditions_1: ionic strength: 30 mM; pH: 7.4; pressure: 1 atm; temperature: 317 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HNCAsample_3isotropicsample_conditions_1
3D HNCACBsample_3isotropicsample_conditions_1
3D HN(CO)CAsample_3isotropicsample_conditions_1
3D HN(COCA)CBsample_3isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Related Database Links:

EMBL CAA64642
GB AAB31698
REF NP_776971 XP_003482237 XP_004408126 XP_005969718 XP_006069254
SP P46065
TPG DAA16514

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts