BMRB Entry 18109

Name: 1H, 13C and 15N resonance assignment of MmpS4
Published: 2012-01-06

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18109

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: 1H, 13C and 15N resonance assignment of MmpS4

Deposition date: 2011-11-29 Original release date: 2012-01-06

Authors: Sun, Peibei; Tian, Changlin

Citation: Sun, Peibei. "1H, 13C and 15N resonance assignment of MmpS4" Biomol. NMR Assignments ., .-..

Assembly members:
MmpS4, polymer, 140 residues, Formula weight is not available

Natural source: Common Name: Mycobacterium tuberculosis Taxonomy ID: 1773 Superkingdom: Bacteria Kingdom: not available Genus/species: Mycobacterium tuberculosis

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
MmpS4: MLMRTWIPLVILVVVIVGGF TVHRIRGFFGSENRPSYSDT NLENSKPFNPKHLTYEIFGP PGTVADISYFDVNSEPQRVD GAVLPWSLHITTNDAAVMGN IVAQGNSDSIGCRITVDGKV RAERVSNEVNAYTYCLVKSA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	227
15N chemical shifts	73
1H chemical shifts	73

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MmpS4	1

Entities:

Entity 1, MmpS4 140 residues - Formula weight is not available

1	MET	LEU	MET	ARG	THR	TRP	ILE	PRO	LEU	VAL
2	ILE	LEU	VAL	VAL	VAL	ILE	VAL	GLY	GLY	PHE
3	THR	VAL	HIS	ARG	ILE	ARG	GLY	PHE	PHE	GLY
4	SER	GLU	ASN	ARG	PRO	SER	TYR	SER	ASP	THR
5	ASN	LEU	GLU	ASN	SER	LYS	PRO	PHE	ASN	PRO
6	LYS	HIS	LEU	THR	TYR	GLU	ILE	PHE	GLY	PRO
7	PRO	GLY	THR	VAL	ALA	ASP	ILE	SER	TYR	PHE
8	ASP	VAL	ASN	SER	GLU	PRO	GLN	ARG	VAL	ASP
9	GLY	ALA	VAL	LEU	PRO	TRP	SER	LEU	HIS	ILE
10	THR	THR	ASN	ASP	ALA	ALA	VAL	MET	GLY	ASN
11	ILE	VAL	ALA	GLN	GLY	ASN	SER	ASP	SER	ILE
12	GLY	CYS	ARG	ILE	THR	VAL	ASP	GLY	LYS	VAL
13	ARG	ALA	GLU	ARG	VAL	SER	ASN	GLU	VAL	ASN
14	ALA	TYR	THR	TYR	CYS	LEU	VAL	LYS	SER	ALA

Samples:

sample_1: MmpS4, [U-99% 13C; U-99% 15N], 0.5 mM; Na2HPO4/NaH2PO4 50 mM; DTT 2 mM

sample_conditions_1: ionic strength: 50 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

Varian INOVA 700 MHz

BMRB	18602
PDB	2LW3
DBJ	BAH24756 BAL64310 BAQ04318
EMBL	CAL70475 CCC25525 CCC42789 CCC63051 CCE35989
GB	AAK44690 ABQ72177 ABR04800 ACT23482 AEB02585
REF	NP_214965 NP_854122 WP_003402272 WP_015456133 WP_016719779
SP	P0A5K3 P9WJS8 P9WJS9

Biological Magnetic Resonance Data Bank