BMRB Entry 18197
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18197
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Title: rat Angiogenin PubMed: 22477091
Deposition date: 2012-01-16 Original release date: 2012-05-11
Authors: Lee, Chung-Kyung; Cheong, Hae-Kap
Citation: Lee, Chung-Kyung; Yeo, Kwon Joo; Hwang, Eunha; Cheong, Hae-Kap. "(1)H, (13)C, (15)N backbone and side-chain resonance assignments of rat angiogenin." Biomol. NMR Assignments 7, 89-92 (2013).
Assembly members:
angiogenin, polymer, 126 residues, Formula weight is not available
Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
angiogenin: GSHMAQDDPRYTKFLTQHYD
AKPKGRDARYCESMMRRRGL
TSPCKEVNTFIHGNKGSIKA
ICGANGSPYGENLRISQSPF
QITTCKHTGGSPRPPCRYRA
SAGFRHVVIACENGLPVHFD
ESFISL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 482 |
| 15N chemical shifts | 123 |
| 1H chemical shifts | 767 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | angiogenin | 1 |
Entities:
Entity 1, angiogenin 126 residues - Formula weight is not available
| 1 | GLY | SER | HIS | MET | ALA | GLN | ASP | ASP | PRO | ARG | ||||
| 2 | TYR | THR | LYS | PHE | LEU | THR | GLN | HIS | TYR | ASP | ||||
| 3 | ALA | LYS | PRO | LYS | GLY | ARG | ASP | ALA | ARG | TYR | ||||
| 4 | CYS | GLU | SER | MET | MET | ARG | ARG | ARG | GLY | LEU | ||||
| 5 | THR | SER | PRO | CYS | LYS | GLU | VAL | ASN | THR | PHE | ||||
| 6 | ILE | HIS | GLY | ASN | LYS | GLY | SER | ILE | LYS | ALA | ||||
| 7 | ILE | CYS | GLY | ALA | ASN | GLY | SER | PRO | TYR | GLY | ||||
| 8 | GLU | ASN | LEU | ARG | ILE | SER | GLN | SER | PRO | PHE | ||||
| 9 | GLN | ILE | THR | THR | CYS | LYS | HIS | THR | GLY | GLY | ||||
| 10 | SER | PRO | ARG | PRO | PRO | CYS | ARG | TYR | ARG | ALA | ||||
| 11 | SER | ALA | GLY | PHE | ARG | HIS | VAL | VAL | ILE | ALA | ||||
| 12 | CYS | GLU | ASN | GLY | LEU | PRO | VAL | HIS | PHE | ASP | ||||
| 13 | GLU | SER | PHE | ILE | SER | LEU |
Samples:
sample_1: angiogenin, [U-100% 13C; U-100% 15N], 1.0 mM; MES 50 mM; sodium chloride 100 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0.1 M; pH: 6.0; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment
TOPSPIN, Bruker Biospin - collection, processing
NMR spectrometers:
- Bruker DRX 500 MHz
Related Database Links:
| PDB | |
| GB | AAR28758 AAV87193 EDL88436 EDL88438 |
| REF | NP_001006993 XP_006251936 |
| TPE | CDG32031 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts