BMRB Entry 18280
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18280
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for CD81LEL PubMed: 22740401
Deposition date: 2012-02-20 Original release date: 2013-02-18
Authors: Berditchevski, Fedor; Sridhar, Pooja; Cocquerel, Laurence; Tews, Birke
Citation: Rajesh, Sundaresan; Sridhar, Pooja; Tews, Birke Andrea; Feneant, Lucie; Cocquerel, Laurence; Ward, Douglas; Berditchevski, Fedor; Overduin, Michael. "Structural basis of ligand interactions of the large extracellular domain of tetraspanin CD81." J. Virol. 86, 9606-9616 (2012).
Assembly members:
CD81_large_extracellular_loop, polymer, 102 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
CD81_large_extracellular_loop: GPLGSGFVNKDQIAKDVKQF
YDQALQQAVVDDDANNAKAV
VKTFHETLDCCGSSTLTALT
TSVLKNNLCPSGSNIISNLF
KEDCHQKIDDLFSGKLHHHH
HH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 257 |
| 15N chemical shifts | 85 |
| 1H chemical shifts | 85 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CD81 LEL | 1 |
Entities:
Entity 1, CD81 LEL 102 residues - Formula weight is not available
Residues 107-111 represent vector sequence after GST cleavage and residues 203-208 are the affinity His-tag sequence
| 1 | GLY | PRO | LEU | GLY | SER | GLY | PHE | VAL | ASN | LYS | ||||
| 2 | ASP | GLN | ILE | ALA | LYS | ASP | VAL | LYS | GLN | PHE | ||||
| 3 | TYR | ASP | GLN | ALA | LEU | GLN | GLN | ALA | VAL | VAL | ||||
| 4 | ASP | ASP | ASP | ALA | ASN | ASN | ALA | LYS | ALA | VAL | ||||
| 5 | VAL | LYS | THR | PHE | HIS | GLU | THR | LEU | ASP | CYS | ||||
| 6 | CYS | GLY | SER | SER | THR | LEU | THR | ALA | LEU | THR | ||||
| 7 | THR | SER | VAL | LEU | LYS | ASN | ASN | LEU | CYS | PRO | ||||
| 8 | SER | GLY | SER | ASN | ILE | ILE | SER | ASN | LEU | PHE | ||||
| 9 | LYS | GLU | ASP | CYS | HIS | GLN | LYS | ILE | ASP | ASP | ||||
| 10 | LEU | PHE | SER | GLY | LYS | LEU | HIS | HIS | HIS | HIS | ||||
| 11 | HIS | HIS |
Samples:
sample_1: CD81 large extracellular loop, [U-99% 13C; U-99% 15N], 1 mM; sodium phosphate 20 mM; sodium chloride 150 mM; AEBSF protease inhibitor 50 uM
sample_conditions_1: ionic strength: 150 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HCACO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
Analysis_CCPN v2.1, Boucher, CCPN, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment, data analysis, data analysis
NMR spectrometers:
- Varian INOVA 800 MHz
- Varian INOVA 900 MHz
Related Database Links:
| PDB | |
| GB | AAA36663 AAD11440 AAH02978 AAH93047 AAV38313 |
| REF | NP_001009023 NP_001284578 NP_004347 XP_003282135 XP_004050511 |
| SP | P60033 P60034 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts