BMRB Entry 18331
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18331
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Title: 1H, 13C and 15N assignments of the holo-acyl carrier protein of Pseudomonas aeruginosa PubMed: 22843382
Deposition date: 2012-03-15 Original release date: 2012-09-14
Authors: Duggan, Brendan; Roca, Amancio; Zhang, Yong-Mei
Citation: Duggan, Brendan; Roca, Amancio; Zhang, Yong-Mei. "1H, 13C and 15N assignments of the holo-acyl carrier protein of Pseudomonas aeruginosa." Biomol. NMR Assignments 7, 225-228 (2013).
Assembly members:
PaAcpP, polymer, 81 residues, 9008.8515 Da.
Natural source: Common Name: Pseudomonas aeruginosa Taxonomy ID: 287 Superkingdom: Bacteria Kingdom: not available Genus/species: Pseudomonas aeruginosa
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
PaAcpP: GSHMSTIEERVKKIVAEQLG
VKEEEVTNSASFVEDLGADS
LDTVELVMALEEEFETEIPD
EKAEKITTVQEAIDYIVAHQ
Q
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 338 |
| 15N chemical shifts | 83 |
| 1H chemical shifts | 563 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PaAcpP | 1 |
Entities:
Entity 1, PaAcpP 81 residues - 9008.8515 Da.
| 1 | GLY | SER | HIS | MET | SER | THR | ILE | GLU | GLU | ARG | ||||
| 2 | VAL | LYS | LYS | ILE | VAL | ALA | GLU | GLN | LEU | GLY | ||||
| 3 | VAL | LYS | GLU | GLU | GLU | VAL | THR | ASN | SER | ALA | ||||
| 4 | SER | PHE | VAL | GLU | ASP | LEU | GLY | ALA | ASP | SER | ||||
| 5 | LEU | ASP | THR | VAL | GLU | LEU | VAL | MET | ALA | LEU | ||||
| 6 | GLU | GLU | GLU | PHE | GLU | THR | GLU | ILE | PRO | ASP | ||||
| 7 | GLU | LYS | ALA | GLU | LYS | ILE | THR | THR | VAL | GLN | ||||
| 8 | GLU | ALA | ILE | ASP | TYR | ILE | VAL | ALA | HIS | GLN | ||||
| 9 | GLN |
Samples:
15N-labelled: PaAcpP, [U-99% 15N], 2.0 mM; sodium phosphate 50.0 mM; sodium chloride 50.0 mM; H2O 5%; D2O 95%
13C-15N-labelled: PaAcpP, [U-99% 13C; U-99% 15N], 2.0 mM; sodium phosphate 50.0 mM; sodium chloride 50.0 mM; H2O 5%; D2O 95%
13C-15N_D2O: PaAcpP, [U-99% 13C; U-99% 15N], 2.0 mM; sodium phosphate 50.0 mM; sodium chloride 50.0 mM; D2O 100%
CondSet1: ionic strength: 0.050 M; pH: 7.000; pressure: 1.000 atm; temperature: 298.000 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC/HMQC | 15N-labelled | isotropic | CondSet1 |
| 3D HNCACB | 13C-15N-labelled | isotropic | CondSet1 |
| cbcaconh ({CA|Cca}coNH) | 13C-15N-labelled | isotropic | CondSet1 |
| 3D HNCO | 13C-15N-labelled | isotropic | CondSet1 |
| hCCconh (C_ccoNH.TOCSY) | 13C-15N-labelled | isotropic | CondSet1 |
| 3D 1H-15N NOESY | 15N-labelled | isotropic | CondSet1 |
| 3D H(CCO)NH | 13C-15N-labelled | isotropic | CondSet1 |
| 2D 1H-13C HSQC/HMQC | 13C-15N_D2O | isotropic | CondSet1 |
| hbCBcgcdHD | 13C-15N_D2O | isotropic | CondSet1 |
| HMBC-HSQC | 13C-15N_D2O | isotropic | CondSet1 |
Software:
ANALYSIS v2.1, CCPN - assignment
TALOS+, Shen Yang - secondary structure prediction
TOPSPIN v2.1, Bruker - acquisition
NMRPipe v2011, Frank Delaglio - processing
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| BMRB | 18821 |
| DBJ | BAK90915 BAP22431 BAP50057 BAQ38943 BAR67004 |
| EMBL | CAW26824 CCQ85094 CDF85073 CDH70383 CDH76426 |
| GB | AAB94392 AAG06354 ABJ12204 ABR81030 AEO74481 |
| REF | NP_251656 WP_003091135 WP_007160597 WP_015477931 |
| SP | O54439 |
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