BMRB Entry 18409
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18409
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Title: 1H Chemical Shift Assignments for the second transmembrane domain from human copper transport 1 PubMed: 22615137
Deposition date: 2012-04-20 Original release date: 2012-05-22
Authors: Yang, Lei; Huang, Zhaowei; Li, Fei
Citation: Yang, Lei; Huang, Zhaowei; Li, Fei. "Structural insights into the transmembrane domains of human copper transporter 1." J. Pept. Sci. 18, 449-455 (2012).
Assembly members:
entity, polymer, 29 residues, 3540.426 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: obtained from a vendor
Entity Sequences (FASTA):
entity: KHLLQTVLHIIQVVISYFLM
LIFMTYNKK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 220 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | second transmembrane domain from human copper transport 1 | 1 |
Entities:
Entity 1, second transmembrane domain from human copper transport 1 29 residues - 3540.426 Da.
| 1 | LYS | HIS | LEU | LEU | GLN | THR | VAL | LEU | HIS | ILE | ||||
| 2 | ILE | GLN | VAL | VAL | ILE | SER | TYR | PHE | LEU | MET | ||||
| 3 | LEU | ILE | PHE | MET | THR | TYR | ASN | LYS | LYS |
Samples:
sample_1: hCtr1-TMD2 2 mM; 40% hexafluoroisopropanol (HFIP) aqueous solution 40%
sample_conditions_1: pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA, Guntert P. - refinement
NMR spectrometers:
- Bruker Avance 500 MHz