BMRB Entry 18479
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18479
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Title: HRas166*GDP backbone chemical shift assignments PubMed: 21814767
Deposition date: 2012-05-24 Original release date: 2013-02-28
Authors: Kovrigin, Evgenii; O'Connor, Casey
Citation: Kovrigin, Casey. "Assignments of backbone H, C and N resonances in H-Ras (1-166) complexed with GppNHp at physiological pH." Biomol. NMR Assignments 6, 91-93 (2012).
Assembly members:
HRas166, polymer, 166 residues, Formula weight is not available
Natural source: Common Name: Humans Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
HRas166: MTEYKLVVVGAGGVGKSALT
IQLIQNHFVDEYDPTIEDSY
RKQVVIDGETCLLDILDTAG
QEEYSAMRDQYMRTGEGFLC
VFAINNTKSFEDIHQYREQI
KRVKDSDDVPMVLVGNKCDL
AARTVESRQAQDLARSYGIP
YIETSAKTRQGVEDAFYTLV
REIRQH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 469 |
| 15N chemical shifts | 161 |
| 1H chemical shifts | 161 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HRas166 | 1 |
Entities:
Entity 1, HRas166 166 residues - Formula weight is not available
| 1 | MET | THR | GLU | TYR | LYS | LEU | VAL | VAL | VAL | GLY | ||||
| 2 | ALA | GLY | GLY | VAL | GLY | LYS | SER | ALA | LEU | THR | ||||
| 3 | ILE | GLN | LEU | ILE | GLN | ASN | HIS | PHE | VAL | ASP | ||||
| 4 | GLU | TYR | ASP | PRO | THR | ILE | GLU | ASP | SER | TYR | ||||
| 5 | ARG | LYS | GLN | VAL | VAL | ILE | ASP | GLY | GLU | THR | ||||
| 6 | CYS | LEU | LEU | ASP | ILE | LEU | ASP | THR | ALA | GLY | ||||
| 7 | GLN | GLU | GLU | TYR | SER | ALA | MET | ARG | ASP | GLN | ||||
| 8 | TYR | MET | ARG | THR | GLY | GLU | GLY | PHE | LEU | CYS | ||||
| 9 | VAL | PHE | ALA | ILE | ASN | ASN | THR | LYS | SER | PHE | ||||
| 10 | GLU | ASP | ILE | HIS | GLN | TYR | ARG | GLU | GLN | ILE | ||||
| 11 | LYS | ARG | VAL | LYS | ASP | SER | ASP | ASP | VAL | PRO | ||||
| 12 | MET | VAL | LEU | VAL | GLY | ASN | LYS | CYS | ASP | LEU | ||||
| 13 | ALA | ALA | ARG | THR | VAL | GLU | SER | ARG | GLN | ALA | ||||
| 14 | GLN | ASP | LEU | ALA | ARG | SER | TYR | GLY | ILE | PRO | ||||
| 15 | TYR | ILE | GLU | THR | SER | ALA | LYS | THR | ARG | GLN | ||||
| 16 | GLY | VAL | GLU | ASP | ALA | PHE | TYR | THR | LEU | VAL | ||||
| 17 | ARG | GLU | ILE | ARG | GLN | HIS |
Samples:
sample_1: HRas166, [U-99% 13C; U-99% 15N], 0.95 ± 0.1 mM; magnesium chloride 5 ± 0.1 mM; GDP 1 ± 0.1 mM; TRIS 10 ± 0.1 mM; sodium chloride 10 ± 0.1 mM; DTT 1 ± 0.1 mM; sodium azide 0.01 ± 0.0001 %; roche inhibitors 0.01 ± 0.001 x; EDTA 0.1 ± 0.01 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 7.2; pressure: 1 atm; temperature: 293.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY v3.115, Goddard - chemical shift assignment, data analysis, peak picking
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| BMRB | 10051 17183 17610 17678 18461 18629 25730 |
| PDB | |
| DBJ | BAB61869 BAB61870 BAB88314 BAB88315 BAB88316 |
| EMBL | CAA25322 CAA25624 CAA27258 CAA35240 CAA90306 |
| GB | AAA36554 AAA42009 AAA46568 AAA46569 AAA46570 |
| PIR | A43816 TVMVNS |
| PRF | 0904302A 1604384A |
| REF | NP_001017003 NP_001018465 NP_001084278 NP_001091711 NP_001123913 |
| SP | P01112 P01113 P01114 P01115 P08642 |
| TPG | DAA13500 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts