BMRB Entry 18581
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18581
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Solution structure of gp78 CUE domain PubMed: 23123110
Deposition date: 2012-07-09 Original release date: 2012-11-19
Authors: Liu, Shan; Chen, Yinghua; Huang, Tao; Tarasov, Sergey; King, Aaren; Li, Jess; Weissman, Allan; Byrd, Robert; Das, Ranabir
Citation: Liu, Shan; Chen, Yinghua; Li, Jess; Huang, Tao; Tarasov, Sergey; King, Aaren; Weissman, Allan; Byrd, R. Andrew; Das, Ranabir. "Promiscuous interactions of gp78 E3 ligase CUE domain with polyubiquitin chains." Structure 20, 2138-2150 (2012).
Assembly members:
entity, polymer, 52 residues, 5966.808 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: SNSQLNAMAHQIQEMFPQVP
YHLVLQDLQLTRSVEITTDN
ILEGRIQVPFPT
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 231 |
| 15N chemical shifts | 53 |
| 1H chemical shifts | 378 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | gp78 CUE domain | 1 |
Entities:
Entity 1, gp78 CUE domain 52 residues - 5966.808 Da.
| 1 | SER | ASN | SER | GLN | LEU | ASN | ALA | MET | ALA | HIS | ||||
| 2 | GLN | ILE | GLN | GLU | MET | PHE | PRO | GLN | VAL | PRO | ||||
| 3 | TYR | HIS | LEU | VAL | LEU | GLN | ASP | LEU | GLN | LEU | ||||
| 4 | THR | ARG | SER | VAL | GLU | ILE | THR | THR | ASP | ASN | ||||
| 5 | ILE | LEU | GLU | GLY | ARG | ILE | GLN | VAL | PRO | PHE | ||||
| 6 | PRO | THR |
Samples:
sample_1: TRIS 50 mM; sodium chloride 50 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 7.2; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aromatic | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment, peak picking
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution
NMR spectrometers:
- Bruker INOVA 600 MHz
- Bruker INOVA 700 MHz
- Varian INOVA 600 MHz
- Varian INOVA 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAE01277 BAE34049 BAE41974 BAK63135 |
| GB | AAD56721 AAD56722 AAH03256 AAH17043 AAH34538 |
| REF | NP_001039439 NP_001135 NP_001267243 NP_035917 XP_001091030 |
| SP | Q9R049 Q9UKV5 |
| TPG | DAA20037 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts