BMRB Entry 18606
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18606
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Title: Solution structure of anti-CRISPR protein Acr30-35 from Pseudomonas aeruginosa Phage JBD30 PubMed: 23242138
Deposition date: 2012-07-22 Original release date: 2013-02-14
Authors: Maxwell, Karen; Bondy-Denomy, Joe; Davidson, Alan
Citation: Bondy-Denomy, Joe; Pawluk, April; Maxwell, Karen; Davidson, Alan. "Bacteriophage genes that inactivate the CRISPR/Cas bacterial immune system." Nature 493, 429-432 (2013).
Assembly members:
entity, polymer, 79 residues, 8825.006 Da.
Natural source: Common Name: g-proteobacteria Taxonomy ID: 1223260 Superkingdom: Bacteria Kingdom: not available Genus/species: Pseudomonas Pseudomonas Bacteriophage JBD30
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: GMKFIKYLSTAHLNYMNIAV
YENGSKIKARVENVVNGKSV
GARDFDSTEQLESWFYGLPG
SGLGRIENAMNEISRRENP
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 239 |
| 15N chemical shifts | 75 |
| 1H chemical shifts | 509 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | anti-CRISPR protein Acr30-35 | 1 |
Entities:
Entity 1, anti-CRISPR protein Acr30-35 79 residues - 8825.006 Da.
| 1 | GLY | MET | LYS | PHE | ILE | LYS | TYR | LEU | SER | THR | ||||
| 2 | ALA | HIS | LEU | ASN | TYR | MET | ASN | ILE | ALA | VAL | ||||
| 3 | TYR | GLU | ASN | GLY | SER | LYS | ILE | LYS | ALA | ARG | ||||
| 4 | VAL | GLU | ASN | VAL | VAL | ASN | GLY | LYS | SER | VAL | ||||
| 5 | GLY | ALA | ARG | ASP | PHE | ASP | SER | THR | GLU | GLN | ||||
| 6 | LEU | GLU | SER | TRP | PHE | TYR | GLY | LEU | PRO | GLY | ||||
| 7 | SER | GLY | LEU | GLY | ARG | ILE | GLU | ASN | ALA | MET | ||||
| 8 | ASN | GLU | ILE | SER | ARG | ARG | GLU | ASN | PRO |
Samples:
sample_1: sodium phosphate 25 mM; sodium chloride 200 mM; H2O 90%; D2O 10%
sample_2: sodium phosphate 25 mM; sodium chloride 200 mM; D2O 100%
sample_conditions_1: ionic strength: 200 mM; pH: 6.8; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aromatic | sample_2 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_2 | isotropic | sample_conditions_1 |
Software:
TALOS, Cornilescu, Delaglio and Bax - data analysis
SPARKY, Goddard - chemical shift assignment, peak picking
NMR spectrometers:
- Varian INOVA 500 MHz
- Varian INOVA 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAR65419 |
| EMBL | CDM55208 CRN29922 CRN44629 CRN56640 CRN59456 |
| GB | AFE86461 AFQ21949 ERX38869 EZO98398 |
| REF | WP_016068276 WP_033975723 YP_006560529 YP_007392342 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts