BMRB Entry 19101
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19101
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Title: Enterocin 7B PubMed: 23725536
Deposition date: 2013-03-18 Original release date: 2013-04-18
Authors: Lohans, Christopher; Towle, Kaitlyn; Miskolzie, Mark; McKay, Ryan; van Belkum, Marco; McMullen, Lynn; Vederas, John
Citation: Lohans, Christopher; Towle, Kaitlyn; Miskolzie, Mark; McKay, Ryan; van Belkum, Marco; McMullen, Lynn; Vederas, John. "Solution Structures of the Linear Leaderless Bacteriocins Enterocin 7A and 7B Resemble Carnocyclin A, a Circular Antimicrobial Peptide" Biochemistry 52, 3987-3994 (2013).
Assembly members:
Enterocin_7B, polymer, 43 residues, 5219.337 Da.
Natural source: Common Name: Firmicutes Taxonomy ID: 1351 Superkingdom: Bacteria Kingdom: not available Genus/species: Enterococcus faecalis
Experimental source: Production method: purified from the natural source Host organism: Enterococcus faecalis
Entity Sequences (FASTA):
Enterocin_7B: XGAIAKLVAKFGWPFIKKFY
KQIMQFIGQGWTIDQIEKWL
KRH
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 299 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Enterocin 7B | 1 |
Entities:
Entity 1, Enterocin 7B 43 residues - 5219.337 Da.
| 1 | FME | GLY | ALA | ILE | ALA | LYS | LEU | VAL | ALA | LYS | ||||
| 2 | PHE | GLY | TRP | PRO | PHE | ILE | LYS | LYS | PHE | TYR | ||||
| 3 | LYS | GLN | ILE | MET | GLN | PHE | ILE | GLY | GLN | GLY | ||||
| 4 | TRP | THR | ILE | ASP | GLN | ILE | GLU | LYS | TRP | LEU | ||||
| 5 | LYS | ARG | HIS |
Samples:
sample_1: Enterocin 7B 0.8 mM; DSS 0.01 w/v; H2O 90%; D2O 10%
sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution
NMR spectrometers:
- Varian VNMRS 700 MHz