BMRB Entry 26549
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26549
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Backbone 1H, 13C and 15N assignment of the intrinsically disordered region of HCV protein NS5A (191-447) PubMed: 6445449
Deposition date: 2015-03-30 Original release date: 2015-04-27
Authors: Solyom, Zsofia; Schwarten, Melanie; Willbold, Dieter; Brutscher, Bernhard
Citation: Solyom, Zsofia; Ma, Peixiang; Schwarten, Melanie; Durand, Gregory; Willbold, Dieter; Brutscher, Bernhard. "Conformational properties of the disordered region of the NS5A protein from hepatitis C virus and their modulation by SH3 binding and phosphorylation" Biophys J. 109, 1483-1496 (2015).
Assembly members:
NS5A(191-447), polymer, 268 residues, Formula weight is not available
Natural source: Common Name: hepatitis C virus Taxonomy ID: 11103 Superkingdom: Viruses Kingdom: not available Genus/species: Hepacivirus hepatitis C virus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
NS5A(191-447): GHMASGSLRGGEPEPDVTVL
TSMLTDPSHITAETAKRRLA
RGSPPSLASSSASQLSAPSL
KATCTTHHDSPDADLIEANL
LWRQEMGGNITRVESENKVV
ILDSFEPLHADGDEREISVA
AEILRKSRKFPSALPIWARP
DYNPPLLESWKDPDYVPPVV
HGCPLPPTKAPPIPPPRRKR
TVVLTESNVSSALAELATKT
FGSSGSSAVDSGTATALPDQ
ASDDGDKGSDVESYSSMPPL
EGEPGDPDLSDGSWSTVSEE
ASEDVVCC
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 689 |
| 15N chemical shifts | 235 |
| 1H chemical shifts | 433 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | NS5A D2D3 | 1 |
Entities:
Entity 1, NS5A D2D3 268 residues - Formula weight is not available
| 1 | GLY | HIS | MET | ALA | SER | GLY | SER | LEU | ARG | GLY | ||||
| 2 | GLY | GLU | PRO | GLU | PRO | ASP | VAL | THR | VAL | LEU | ||||
| 3 | THR | SER | MET | LEU | THR | ASP | PRO | SER | HIS | ILE | ||||
| 4 | THR | ALA | GLU | THR | ALA | LYS | ARG | ARG | LEU | ALA | ||||
| 5 | ARG | GLY | SER | PRO | PRO | SER | LEU | ALA | SER | SER | ||||
| 6 | SER | ALA | SER | GLN | LEU | SER | ALA | PRO | SER | LEU | ||||
| 7 | LYS | ALA | THR | CYS | THR | THR | HIS | HIS | ASP | SER | ||||
| 8 | PRO | ASP | ALA | ASP | LEU | ILE | GLU | ALA | ASN | LEU | ||||
| 9 | LEU | TRP | ARG | GLN | GLU | MET | GLY | GLY | ASN | ILE | ||||
| 10 | THR | ARG | VAL | GLU | SER | GLU | ASN | LYS | VAL | VAL | ||||
| 11 | ILE | LEU | ASP | SER | PHE | GLU | PRO | LEU | HIS | ALA | ||||
| 12 | ASP | GLY | ASP | GLU | ARG | GLU | ILE | SER | VAL | ALA | ||||
| 13 | ALA | GLU | ILE | LEU | ARG | LYS | SER | ARG | LYS | PHE | ||||
| 14 | PRO | SER | ALA | LEU | PRO | ILE | TRP | ALA | ARG | PRO | ||||
| 15 | ASP | TYR | ASN | PRO | PRO | LEU | LEU | GLU | SER | TRP | ||||
| 16 | LYS | ASP | PRO | ASP | TYR | VAL | PRO | PRO | VAL | VAL | ||||
| 17 | HIS | GLY | CYS | PRO | LEU | PRO | PRO | THR | LYS | ALA | ||||
| 18 | PRO | PRO | ILE | PRO | PRO | PRO | ARG | ARG | LYS | ARG | ||||
| 19 | THR | VAL | VAL | LEU | THR | GLU | SER | ASN | VAL | SER | ||||
| 20 | SER | ALA | LEU | ALA | GLU | LEU | ALA | THR | LYS | THR | ||||
| 21 | PHE | GLY | SER | SER | GLY | SER | SER | ALA | VAL | ASP | ||||
| 22 | SER | GLY | THR | ALA | THR | ALA | LEU | PRO | ASP | GLN | ||||
| 23 | ALA | SER | ASP | ASP | GLY | ASP | LYS | GLY | SER | ASP | ||||
| 24 | VAL | GLU | SER | TYR | SER | SER | MET | PRO | PRO | LEU | ||||
| 25 | GLU | GLY | GLU | PRO | GLY | ASP | PRO | ASP | LEU | SER | ||||
| 26 | ASP | GLY | SER | TRP | SER | THR | VAL | SER | GLU | GLU | ||||
| 27 | ALA | SER | GLU | ASP | VAL | VAL | CYS | CYS |
Samples:
sample_1: NS5A(191-447), [U-100% 13C; U-100% 15N], 120 uM; D2O, [U-100% 2H], 10%; potassium phosphate 50 mM; sodium chloride 20 mM; TCEP 2 mM
sample_conditions_1: ionic strength: 0.32 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N BEST-TROSY | sample_1 | isotropic | sample_conditions_1 |
| 3D BT-HNCOCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D BT-iHNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D BT-hNcocaNH | sample_1 | isotropic | sample_conditions_1 |
| 3D BT-hnCOcaNH | sample_1 | isotropic | sample_conditions_1 |
Software:
VNMRJ, Varian - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
Analysis, CCPN - chemical shift assignment
NMR spectrometers:
- Varian INOVA 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts