BMRB Entry 4093
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4093
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Title: Secondary Structure of the Ribonuclease H Domain of the Human Immunodeficiency Virus Reverse Transcriptase in Solution Using Three-Dimensional Double and Triple Resonance Heteronuclear Magnetic Resonance Spectroscopy
Deposition date: 1998-01-08 Original release date: 1998-03-02
Authors: Powers, Robert; Clore, G.; Bax, Ad; Garrett, Daniel; Stahl, Stephen; Wingfield, Paul; Gronenborn, Angela
Citation: Powers, Robert; Clore, G.; Bax, Ad; Garrett, Daniel; Stahl, Stephen; Wingfield, Paul; Gronenborn, Angela. "Secondary Structure of the Ribonuclease H Domain of the Human Immunodeficiency Virus Reverse Transcriptase in Solution Using Three-Dimensional Double and Triple Resonance Heteronuclear Magnetic Resonance Spectroscopy" J. Biomol. NMR 221, 1081-1090 (1991).
Assembly members:
Reverse Transcriptase of HIV-1, polymer, 138 residues, Formula weight is not available
Natural source: Common Name: HIV-1 Taxonomy ID: 11706 Superkingdom: not available Kingdom: not available Genus/species: Lentivirus HIV-1
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Reverse Transcriptase of HIV-1: MNELYQLEKEPIVGAETFYV
DGAANRETKLGKAGYVTNRG
RQKVVTLTDTTNQKTELQAI
YLALQDSGLEVNIVTDSQYA
LGIIQAQPDQSESELVNQII
EQLIKKEKVYLAAVPAHKGI
GGNEQVDKLVSAGIRKVL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 239 |
| 15N chemical shifts | 117 |
| 1H chemical shifts | 243 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RNase_H-HIV-1 | 1 |
Entities:
Entity 1, RNase_H-HIV-1 138 residues - Formula weight is not available
| 1 | MET | ASN | GLU | LEU | TYR | GLN | LEU | GLU | LYS | GLU | ||||
| 2 | PRO | ILE | VAL | GLY | ALA | GLU | THR | PHE | TYR | VAL | ||||
| 3 | ASP | GLY | ALA | ALA | ASN | ARG | GLU | THR | LYS | LEU | ||||
| 4 | GLY | LYS | ALA | GLY | TYR | VAL | THR | ASN | ARG | GLY | ||||
| 5 | ARG | GLN | LYS | VAL | VAL | THR | LEU | THR | ASP | THR | ||||
| 6 | THR | ASN | GLN | LYS | THR | GLU | LEU | GLN | ALA | ILE | ||||
| 7 | TYR | LEU | ALA | LEU | GLN | ASP | SER | GLY | LEU | GLU | ||||
| 8 | VAL | ASN | ILE | VAL | THR | ASP | SER | GLN | TYR | ALA | ||||
| 9 | LEU | GLY | ILE | ILE | GLN | ALA | GLN | PRO | ASP | GLN | ||||
| 10 | SER | GLU | SER | GLU | LEU | VAL | ASN | GLN | ILE | ILE | ||||
| 11 | GLU | GLN | LEU | ILE | LYS | LYS | GLU | LYS | VAL | TYR | ||||
| 12 | LEU | ALA | ALA | VAL | PRO | ALA | HIS | LYS | GLY | ILE | ||||
| 13 | GLY | GLY | ASN | GLU | GLN | VAL | ASP | LYS | LEU | VAL | ||||
| 14 | SER | ALA | GLY | ILE | ARG | LYS | VAL | LEU |
Samples:
sample_one: Reverse Transcriptase of HIV-1, [U-95% 13C; U-95% 15N], 1.1 mM; H2O 90%; D2O 10%; Sodium phosphate buffer 100 mM
sample_conditions: pH: 5.4; temperature: 299 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
No software information available
NMR spectrometers:
- Bruker AM600 600 MHz
Related Database Links:
| PDB | |
| GB | AAA93161 AAB50259 AAC55949 AAC55950 AAC82598 |
| REF | NP_057849 NP_705927 NP_789740 YP_001856242 |
| SP | P04585 |
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