BMRB Entry 4097
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4097
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Title: Backbone 1H, 13C and 15N Chemical Shift Assignment for the HIV-1 RNase H Domain
Deposition date: 1998-01-16 Original release date: 1999-01-25
Authors: Gunther, Kern; Handel, Tracy; Marqusee, Susan
Citation: Gunther, Kern; Handel, Tracy; Marqusee, Susan. "Characterization of a Folding Intermediate from HIV-1 Ribonuclease H" Protein Sci. 7, 2164-2174 (1998).
Assembly members:
Reverse Transcriptase of HIV-1, polymer, 135 residues, 14885 Da.
Natural source: Common Name: HIV-1 Taxonomy ID: 11678 Superkingdom: not available Kingdom: not available Genus/species: Lentivirus HIV-1
Experimental source: Production method: chemical construct
Entity Sequences (FASTA):
Reverse Transcriptase of HIV-1: MYQLEKEPIVGAETFYVDGA
ANRETKLGKAGYVTNKGRQK
VVPLTNTTNQKTELQAIYLA
LQDSGLEVNIVTDSQYALGI
IQAQPDKSESELVNQIIEQL
IKKEKVYLAWVPAHKGIGGN
EQVDKLVSAGIRKIL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 217 |
| 15N chemical shifts | 111 |
| 1H chemical shifts | 111 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RNase_H-HIV-1_(RVT) | 1 |
Entities:
Entity 1, RNase_H-HIV-1_(RVT) 135 residues - 14885 Da.
| 1 | MET | TYR | GLN | LEU | GLU | LYS | GLU | PRO | ILE | VAL | ||||
| 2 | GLY | ALA | GLU | THR | PHE | TYR | VAL | ASP | GLY | ALA | ||||
| 3 | ALA | ASN | ARG | GLU | THR | LYS | LEU | GLY | LYS | ALA | ||||
| 4 | GLY | TYR | VAL | THR | ASN | LYS | GLY | ARG | GLN | LYS | ||||
| 5 | VAL | VAL | PRO | LEU | THR | ASN | THR | THR | ASN | GLN | ||||
| 6 | LYS | THR | GLU | LEU | GLN | ALA | ILE | TYR | LEU | ALA | ||||
| 7 | LEU | GLN | ASP | SER | GLY | LEU | GLU | VAL | ASN | ILE | ||||
| 8 | VAL | THR | ASP | SER | GLN | TYR | ALA | LEU | GLY | ILE | ||||
| 9 | ILE | GLN | ALA | GLN | PRO | ASP | LYS | SER | GLU | SER | ||||
| 10 | GLU | LEU | VAL | ASN | GLN | ILE | ILE | GLU | GLN | LEU | ||||
| 11 | ILE | LYS | LYS | GLU | LYS | VAL | TYR | LEU | ALA | TRP | ||||
| 12 | VAL | PRO | ALA | HIS | LYS | GLY | ILE | GLY | GLY | ASN | ||||
| 13 | GLU | GLN | VAL | ASP | LYS | LEU | VAL | SER | ALA | GLY | ||||
| 14 | ILE | ARG | LYS | ILE | LEU |
Samples:
sample_one: Reverse Transcriptase of HIV-1, [U-99.8% 13C; U-99.9% 15N], 1.1 mM
sample_conditions_one: ionic strength: 0.1; pH: 4.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | sample_one | not available | not available |
| HNCA | sample_one | not available | not available |
| CBCA(CO)NH | sample_one | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker DMX600 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAA12989 |
| GB | AAA44198 AAB24839 AAL28068 AAL29457 ADW78894 |
| PRF | 1102247C 1103299E |
| SP | P03366 P0C6F2 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts