BMRB Entry 4165
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4165
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Title: 1H, 13C, and 15N Chemical Shift Assignments for Tn916 N-terminal DNA Binding Domain complex with DNA PubMed: 10382314
Deposition date: 1998-07-16 Original release date: 2000-03-16
Authors: Wojciak, Jonathan; Connolly, Kevin; Clubb, Robert
Citation: Connolly, Kevin; Wojciak, Jonathan; Clubb, Robert. "Resonance assignments of the Tn916 integrase DNA-binding domain and the integrase:DNA complex" J. Biomol. NMR 14, 95-96 (1999).
Assembly members:
Tn916 integrase, polymer, 74 residues, Formula weight is not available
DNA binding site of Tn916 integrase, polymer, 13 residues, Formula weight is not available
DNA binding site of Tn916 integrase, polymer, 13 residues, Formula weight is not available
Natural source: Common Name: Enterococcus faecalis Taxonomy ID: 1351 Superkingdom: Eubacteria Kingdom: not available Genus/species: Enterococcus faecalis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Tn916 integrase: MSEKRRDNRGRILKTGESQR
KDGRYLYKYIDSFGEPQFVY
SWKLVATDRVPAGKRDAISL
REKIAELQKDIHDG
DNA binding site of Tn916 integrase: GAGTAGTAAATTC
DNA binding site of Tn916 integrase: GAATTTACTACTC
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 231 |
| 15N chemical shifts | 80 |
| 1H chemical shifts | 667 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Tn916int | 1 |
| 2 | DNA 1 | 2 |
| 3 | DNA 2 | 3 |
Entities:
Entity 1, Tn916int 74 residues - Formula weight is not available
| 1 | MET | SER | GLU | LYS | ARG | ARG | ASP | ASN | ARG | GLY | ||||
| 2 | ARG | ILE | LEU | LYS | THR | GLY | GLU | SER | GLN | ARG | ||||
| 3 | LYS | ASP | GLY | ARG | TYR | LEU | TYR | LYS | TYR | ILE | ||||
| 4 | ASP | SER | PHE | GLY | GLU | PRO | GLN | PHE | VAL | TYR | ||||
| 5 | SER | TRP | LYS | LEU | VAL | ALA | THR | ASP | ARG | VAL | ||||
| 6 | PRO | ALA | GLY | LYS | ARG | ASP | ALA | ILE | SER | LEU | ||||
| 7 | ARG | GLU | LYS | ILE | ALA | GLU | LEU | GLN | LYS | ASP | ||||
| 8 | ILE | HIS | ASP | GLY |
Entity 2, DNA 1 13 residues - Formula weight is not available
| 1 | DG | DA | DG | DT | DA | DG | DT | DA | DA | DA | ||||
| 2 | DT | DT | DC |
Entity 3, DNA 2 13 residues - Formula weight is not available
| 1 | DG | DA | DA | DT | DT | DT | DA | DC | DT | DA | ||||
| 2 | DC | DT | DC |
Samples:
sample_one: Tn916 integrase 1.0 mM; DNA binding site of Tn916 integrase 1.0 mM; DNA binding site of Tn916 integrase 1.0 mM
sample_conditions_one: pH: 6.0; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| not available | sample_one | not available | sample_conditions_one |
Software:
PIPP - peak assignment
NMR spectrometers:
- Bruker DRX 500 MHz
- Bruker DRX 600 MHz
Related Database Links:
| BMRB | 4160 |
| PDB | |
| DBJ | BAG12510 BAK28444 BAN28951 GAD37680 |
| EMBL | CAA43360 CAO82956 CAQ49380 CAV31149 CAZ51601 |
| GB | AAA19427 AAB60030 AAC36982 AAM99801 AAY63950 |
| PRF | 2114402Y |
| REF | NP_687929 WP_001291559 WP_001291560 WP_001291561 WP_001291562 |
| SP | P22886 P62904 P62905 |
Download simulated HSQC data in one of the following formats:
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or all simulated shifts