BMRB Entry 4174
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4174
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Title: Chemical Shift Assignments of the Mg2+-Loaded State of the N-Terminal Domain of Calmodulin
Deposition date: 1998-08-11 Original release date: 2000-04-03
Authors: Malmendal, Anders; Evenas, Johan; Thulin, Eva; Gippert, Garry; Drakenberg, Torbjorn; Forsen, Sture
Citation: Malmendal, Anders; Evenas, Johan; Thulin, Eva; Gippert, Garry; Drakenberg, Torbjorn; Forsen, Sture. "When Size is Important: Accommodation of Magnesium in a Calcium Binding Regulatory Domain" J. Biol. Chem. 273, 28994-29001 (1998).
Assembly members:
N-terminal domain of calmodulin, polymer, 81 residues, Formula weight is not available
MG, non-polymer, 24.305 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: .
Entity Sequences (FASTA):
N-terminal domain of calmodulin: MADQLTEEQIAEFKEAFSLF
DKDGDGTITTKELGTVMRSL
GQNPTEAELQDMINEVDADG
NGTIDFPEFLTMMARKMKDT
D
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 84 |
| 1H chemical shifts | 531 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TR1C | 1 |
| 2 | Mg | 2 |
Entities:
Entity 1, TR1C 81 residues - Formula weight is not available
| 1 | MET | ALA | ASP | GLN | LEU | THR | GLU | GLU | GLN | ILE | ||||
| 2 | ALA | GLU | PHE | LYS | GLU | ALA | PHE | SER | LEU | PHE | ||||
| 3 | ASP | LYS | ASP | GLY | ASP | GLY | THR | ILE | THR | THR | ||||
| 4 | LYS | GLU | LEU | GLY | THR | VAL | MET | ARG | SER | LEU | ||||
| 5 | GLY | GLN | ASN | PRO | THR | GLU | ALA | GLU | LEU | GLN | ||||
| 6 | ASP | MET | ILE | ASN | GLU | VAL | ASP | ALA | ASP | GLY | ||||
| 7 | ASN | GLY | THR | ILE | ASP | PHE | PRO | GLU | PHE | LEU | ||||
| 8 | THR | MET | MET | ALA | ARG | LYS | MET | LYS | ASP | THR | ||||
| 9 | ASP |
Entity 2, Mg - Mg - 24.305 Da.
| 1 | MG |
Samples:
sample_one: N-terminal domain of calmodulin 4.0 mM; D2O 10%; H2O 90%; KCL 10 mM; Azide 100 uM
sample_two: N-terminal domain of calmodulin, [U-15N], 4.0 mM; D2O 10%; H2O 90%; KCL 10 mM; Azide 100 uM
sample_conditions_one: ionic strength: 0.1; pH: 7.2; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| not available | not available | not available | sample_conditions_one |
Software:
No software information available
NMR spectrometers:
- unknown unknown 0 MHz
Related Database Links:
| BMRB | 18082 18302 |
| PDB | |
| EMBL | CAA29381 CAB65357 CCD82979 CUA69889 |
| GB | AAH53790 ABY16748 ABY16749 ABY16752 ACH46202 |
| REF | XP_001869424 XP_001995129 XP_002772218 XP_006032607 XP_006137175 |
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