BMRB Entry 4215
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4215
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Title: Staphylokinase (NMR, Sakstar Variant)
Deposition date: 1998-06-08 Original release date: 2000-10-02
Authors: Ohlenschlager, Oliver; Ramachandran, Ramadurai; Guhrs, Karl; Schlott, Bernhard; Brown, Larry
Citation: Ohlenschlager, Oliver; Ramachandran, Ramadurai; Guhrs, Karl; Schlott, Bernhard; Brown, Larry. "NMR Solution Structure of the Plasminogen-Activator Protein Staphylokinase" Biochemistry 37, 10635-10642 (1998).
Assembly members:
Staphylokinase, polymer, 136 residues, 15500 Da.
Natural source: Common Name: S. aureus Taxonomy ID: 1280 Superkingdom: Eubacteria Kingdom: not available Genus/species: Staphylococcus aureus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Staphylokinase: SSSFDKGKYKKGDDASYFEP
TGPYLMVNVTGVDSKGNELL
SPHYVEFPIKPGTTLTKEKI
EYYVEWALDATAYKEFRVVE
LDPSAKIEVTYYDKNKKKEE
TKSFPITEKGFVVPDLSEHI
KNPGFNLITKVVIEKK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 629 |
| 15N chemical shifts | 130 |
| 1H chemical shifts | 987 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Staphylokinase | 1 |
Entities:
Entity 1, Staphylokinase 136 residues - 15500 Da.
| 1 | SER | SER | SER | PHE | ASP | LYS | GLY | LYS | TYR | LYS | ||||
| 2 | LYS | GLY | ASP | ASP | ALA | SER | TYR | PHE | GLU | PRO | ||||
| 3 | THR | GLY | PRO | TYR | LEU | MET | VAL | ASN | VAL | THR | ||||
| 4 | GLY | VAL | ASP | SER | LYS | GLY | ASN | GLU | LEU | LEU | ||||
| 5 | SER | PRO | HIS | TYR | VAL | GLU | PHE | PRO | ILE | LYS | ||||
| 6 | PRO | GLY | THR | THR | LEU | THR | LYS | GLU | LYS | ILE | ||||
| 7 | GLU | TYR | TYR | VAL | GLU | TRP | ALA | LEU | ASP | ALA | ||||
| 8 | THR | ALA | TYR | LYS | GLU | PHE | ARG | VAL | VAL | GLU | ||||
| 9 | LEU | ASP | PRO | SER | ALA | LYS | ILE | GLU | VAL | THR | ||||
| 10 | TYR | TYR | ASP | LYS | ASN | LYS | LYS | LYS | GLU | GLU | ||||
| 11 | THR | LYS | SER | PHE | PRO | ILE | THR | GLU | LYS | GLY | ||||
| 12 | PHE | VAL | VAL | PRO | ASP | LEU | SER | GLU | HIS | ILE | ||||
| 13 | LYS | ASN | PRO | GLY | PHE | ASN | LEU | ILE | THR | LYS | ||||
| 14 | VAL | VAL | ILE | GLU | LYS | LYS |
Samples:
sample_one: Staphylokinase, [U-100% 15N], 1.10 mM
sample_two: Staphylokinase, [U-100% 13C;U-100% 15N], 1.28 mM
sample_conditions: pH: 6.7; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N-1H HSQC | not available | not available | sample_conditions |
| 13C-1H HSQC | not available | not available | sample_conditions |
| 1H-15N-1H TOCSY-HSQC | not available | not available | sample_conditions |
| 1H-15N-1H NOESY-HSQC | not available | not available | sample_conditions |
| 1H-15N-1H HBHA(CBCACO)NH | not available | not available | sample_conditions |
| 15N-1H-1H HNHA | not available | not available | sample_conditions |
| 15N-15N-1H HSQC-NOESY-HSQC | not available | not available | sample_conditions |
| 1H-13C-1H HCCH-TOCSY | not available | not available | sample_conditions |
| 1H-13C-1H NOESY-HSQC | not available | not available | sample_conditions |
| 15N-13C-1H HNCO | not available | not available | sample_conditions |
| 15N-13C-1H HNCACB | not available | not available | sample_conditions |
| 13C-15N-1H CBCA(CO)NH | not available | not available | sample_conditions |
| 13C-15N-1H CC(CO)NH | not available | not available | sample_conditions |
| 13C-1H-15N-1H HMQC-NOESY-HSQC | not available | not available | sample_conditions |
| 13C-1H-13C-1H HMQC-NOESY-HSQC | not available | not available | sample_conditions |
Software:
XEASY v1.3.9 - Visualization & bookkeeping, assignment
NMR spectrometers:
- Varian Inova 750 MHz
Related Database Links:
| PDB | |
| DBJ | BAA95011 BAB43032 BAB58106 BAB95750 BAF68152 |
| EMBL | CAA24957 CAA29822 CAG41024 CAG43674 CBI49812 |
| GB | AAA98206 AAB84174 AAK11636 AAL82378 AAP86605 |
| PIR | PRSAK |
| REF | NP_803403 NP_835575 WP_000919348 WP_000919349 WP_000919350 |
| SP | P15240 P68801 P68802 Q6G7Z1 Q6GFB2 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts