BMRB Entry 4257
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4257
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Title: (52-96)C-Terminal Domain of the HIV-1 Regulatory Protein VPR
Deposition date: 1998-10-28 Original release date: 1999-11-23
Authors: Schuler, W.; de Rocquigny, H.; Baudat, Y.; Sire, J.; Roques, B.
Citation: Schuler, W.; Wecker, K.; de Rocquigny, H.; Baudat, Y.; Sire, J.; Roques, B.. "NMR Structure of the (52-96) C-Terminal Domain of the HIV-1 Regulatory Protein Vpr : Molecular Insights into its Biological Functions" J. Mol. Biol. 285, 2105-2117 (1999).
Assembly members:
Vpr_HIV-1_(LAI), polymer, 45 residues, Formula weight is not available
Natural source: Common Name: HIV-1 Taxonomy ID: 11706 Superkingdom: not available Kingdom: not available Genus/species: Lentivirus HIV-1
Experimental source: Production method: chemically synthesized
Entity Sequences (FASTA):
Vpr_HIV-1_(LAI): DTWTGVEALIRILQQLLFIH
FRIGCRHSRIGIIQQRRTRN
GASKS
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 1H chemical shifts | 377 |
| coupling constants | 26 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Vpr_HIV-1_(LAI) | 1 |
Entities:
Entity 1, Vpr_HIV-1_(LAI) 45 residues - Formula weight is not available
| 1 | ASP | THR | TRP | THR | GLY | VAL | GLU | ALA | LEU | ILE | ||||
| 2 | ARG | ILE | LEU | GLN | GLN | LEU | LEU | PHE | ILE | HIS | ||||
| 3 | PHE | ARG | ILE | GLY | CYS | ARG | HIS | SER | ARG | ILE | ||||
| 4 | GLY | ILE | ILE | GLN | GLN | ARG | ARG | THR | ARG | ASN | ||||
| 5 | GLY | ALA | SER | LYS | SER |
Samples:
sample_one: Vpr_HIV-1_(LAI) 2.0 mM; DTT 10.0 mM; TFE, [U-2H], 4.1 M; D2O 5.6 M; H2O 33.3 M; TMSP 0.1 mM
sample_conditions_one: pH: 3.4; temperature: 313 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NOESY | sample_one | not available | sample_conditions_one |
| TOCSY | sample_one | not available | sample_conditions_one |
| E.COSY | sample_one | not available | sample_conditions_one |
Software:
UXNMR - Data acquisition, Processing
FELIX - Data evaluation, Data processing, Structure calculations
NMRchitect - Data evaluation, Data processing, Structure calculations
INSIGHTII - Data evaluation, Data processing, Structure calculations
NMR spectrometers:
- Bruker AMX 600 MHz