BMRB Entry 4307
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4307
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Title: 1H, 13C, and 15N Chemical Shift Assignments for SHa rPrP(90-231). PubMed: 10220323
Deposition date: 1999-02-16 Original release date: 2000-05-10
Authors: James, Thomas; Liu, He; Ulyanov, Nikolai; Far-Jones, Shauna
Citation: Liu, H.; Farr-Jones, S.; Ulyanov, N.; Llinas, M.; Marqusee, S.; Groth, D.; Cohen, F.; Prusiner, S.; James, T.. "Solution structure of Syrian hamster prion protein rPrP(90-231)" Biochemistry 38, 5362-5377 (1999).
Assembly members:
recombinant Syrian hamster prion protein, polymer, 142 residues, Formula weight is not available
Natural source: Common Name: Syrian hamster Taxonomy ID: 10036 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Microcricetus aureus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
recombinant Syrian hamster prion protein: GQGGGTHNQWNKPSKPKTNM
KHMAGAAAAGAVVGGLGGYM
LGSAMSRPMMHFGNDWEDRY
YRENMNRYPNQVYYRPVDQY
NNQNNFVHDCVNITIKQHTV
TTTTKGENFTETDIKIMERV
VEQMCTTQYQKESQAYYDGR
RS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 401 |
| 15N chemical shifts | 168 |
| 1H chemical shifts | 862 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | rPrP(90-231) | 1 |
Entities:
Entity 1, rPrP(90-231) 142 residues - Formula weight is not available
| 1 | GLY | GLN | GLY | GLY | GLY | THR | HIS | ASN | GLN | TRP | ||||
| 2 | ASN | LYS | PRO | SER | LYS | PRO | LYS | THR | ASN | MET | ||||
| 3 | LYS | HIS | MET | ALA | GLY | ALA | ALA | ALA | ALA | GLY | ||||
| 4 | ALA | VAL | VAL | GLY | GLY | LEU | GLY | GLY | TYR | MET | ||||
| 5 | LEU | GLY | SER | ALA | MET | SER | ARG | PRO | MET | MET | ||||
| 6 | HIS | PHE | GLY | ASN | ASP | TRP | GLU | ASP | ARG | TYR | ||||
| 7 | TYR | ARG | GLU | ASN | MET | ASN | ARG | TYR | PRO | ASN | ||||
| 8 | GLN | VAL | TYR | TYR | ARG | PRO | VAL | ASP | GLN | TYR | ||||
| 9 | ASN | ASN | GLN | ASN | ASN | PHE | VAL | HIS | ASP | CYS | ||||
| 10 | VAL | ASN | ILE | THR | ILE | LYS | GLN | HIS | THR | VAL | ||||
| 11 | THR | THR | THR | THR | LYS | GLY | GLU | ASN | PHE | THR | ||||
| 12 | GLU | THR | ASP | ILE | LYS | ILE | MET | GLU | ARG | VAL | ||||
| 13 | VAL | GLU | GLN | MET | CYS | THR | THR | GLN | TYR | GLN | ||||
| 14 | LYS | GLU | SER | GLN | ALA | TYR | TYR | ASP | GLY | ARG | ||||
| 15 | ARG | SER |
Samples:
sample_1: recombinant Syrian hamster prion protein, [U-13C; U-15N], 0.7 – 1.3 mM
cond._set_1: pH: 5.2; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCA | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| HNCO | not available | not available | not available |
| HBHA(CO)NH | not available | not available | not available |
| C(CO)NH-TOCSY | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
| 1H-15N NOESY-HSQC | not available | not available | not available |
Software:
SPARKY v3.6 - anotation, assignment, bookkeeping, peak picking
NMR spectrometers:
- Bruker AMX 500 MHz
- Bruker DRX 600 MHz
- Bruker RMX 750 MHz
- Varian Unity Plus 600 MHz
Related Database Links:
| BMRB | 17034 17834 |
| PDB | |
| GB | AAA37090 AAA37091 AAA37092 AAA37093 AAB25731 |
| REF | XP_005068717 XP_012967855 |
| SP | P04273 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts