BMRB Entry 4322
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4322
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Title: 1H Chemical Shift Assignment for the Complete Consensus V3 Loop Peptide of the Envelope Protein gp120 of HIV-1 in 20% Trifluoroethanol/Water.
Deposition date: 1999-03-19 Original release date: 2007-03-26
Authors: Vranken, Wim; Budesinsky, Milos; Fant, Franky; Boulez, Kris; Borremans, Frans
Citation: Vranken, Wim; Budesinsky, Milos; Fant, Franky; Boulez, Kris; Borremans, Frans. "The Complete Consensus V3 Loop Peptide of the Envelope Protein gp120 of HIV-1 Shows Pronounced Helical Character in Solution." FEBS Lett. 374, 117-121 (1995).
Assembly members:
consensus V3 loop, polymer, 35 residues, 3895.3 Da.
Natural source: Common Name: HIV-1 Taxonomy ID: 11676 Superkingdom: not available Kingdom: not available Genus/species: Lentivirus Human immunodeficiency virus type 1
Experimental source: Production method: vendor
Entity Sequences (FASTA):
consensus V3 loop: CTRPNNNTRKSIHIGPGRAF
YTTGEIIGDIRQAHC
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 480 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Consensus V3 loop | 1 |
Entities:
Entity 1, Consensus V3 loop 35 residues - 3895.3 Da.
| 1 | CYS | THR | ARG | PRO | ASN | ASN | ASN | THR | ARG | LYS | ||||
| 2 | SER | ILE | HIS | ILE | GLY | PRO | GLY | ARG | ALA | PHE | ||||
| 3 | TYR | THR | THR | GLY | GLU | ILE | ILE | GLY | ASP | ILE | ||||
| 4 | ARG | GLN | ALA | HIS | CYS |
Samples:
water_sample: consensus V3 loop 4.0 mM; H2O 90%; D2O 10%
20%_TFE_sample: consensus V3 loop 3.4 mM; TFE 20%; H2O 70%; D2O 10%
lowT: pH: 2.5 na; temperature: 275 K
highT: pH: 2.5 na; temperature: 290 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H magnitude COSY | not available | not available | not available |
| 1H TOCSY | not available | not available | not available |
| 1H NOESY | not available | not available | not available |
| 1H ROESY | not available | not available | not available |
| 1H CT-NOE spectra | not available | not available | not available |
Software:
PRONTO - Analysis of spectra
UXNMR - Processing of spectra
NMR spectrometers:
- Bruker AMX 500 MHz