BMRB Entry 4344
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4344
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Title: 1H and 15N Chemical Shift Assignments for Apo-CopZ
Deposition date: 1999-05-06 Original release date: 1999-05-25
Authors: Wimmer, Reinhard; Herrmann, Torsten; Solioz, Marc; Wuethrich, Kurt
Citation: Wimmer, Reinhard; Herrmann, Torsten; Solioz, Marc; Wuethrich, Kurt. "NMR Structure and Metal Interactions of the CopZ Copper Chaperone" J. Biol. Chem. ., .-..
Assembly members:
CopZ, polymer, 68 residues, Formula weight is not available
Natural source: Common Name: Enterococcus hirae Taxonomy ID: 1354 Superkingdom: Eubacteria Kingdom: not available Genus/species: Enterococcus hirae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
CopZ: AQEFSVKGMSCNHCVARIEE
AVGRISGVKKVKVQLKKEKA
VVKFDEANVQATEICQAINE
LGYQAEVI
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 74 |
| 1H chemical shifts | 443 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | apo CopZ monomer | 1 |
Entities:
Entity 1, apo CopZ monomer 68 residues - Formula weight is not available
| 1 | ALA | GLN | GLU | PHE | SER | VAL | LYS | GLY | MET | SER | ||||
| 2 | CYS | ASN | HIS | CYS | VAL | ALA | ARG | ILE | GLU | GLU | ||||
| 3 | ALA | VAL | GLY | ARG | ILE | SER | GLY | VAL | LYS | LYS | ||||
| 4 | VAL | LYS | VAL | GLN | LEU | LYS | LYS | GLU | LYS | ALA | ||||
| 5 | VAL | VAL | LYS | PHE | ASP | GLU | ALA | ASN | VAL | GLN | ||||
| 6 | ALA | THR | GLU | ILE | CYS | GLN | ALA | ILE | ASN | GLU | ||||
| 7 | LEU | GLY | TYR | GLN | ALA | GLU | VAL | ILE |
Samples:
sample_1: CopZ, [U-15N], 1.3 mM; sodium phosphate 50 mM; Na2S2O4 10 uM
conditions_1: pH: 7.0 na; temperature: 288.2 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 2QF-COSY | not available | not available | not available |
| 2D 3QF-COSY | not available | not available | not available |
| 2D 1H-1H TOCSY | not available | not available | not available |
| 2D 1H-1H NOESY | not available | not available | not available |
| 2D 1H-15N HSQC | not available | not available | not available |
| 3D 1H-1H-15N NOESY | not available | not available | not available |
| 3D 1H-1H-15N TOCSY | not available | not available | not available |
| 3D ct-HNHB | not available | not available | not available |
| mixing time of NOESY spectra: 50 ms | not available | not available | not available |
| mixing time of TOCSY spectra: 50 and 90 ms | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker DRX 500 MHz
- Bruker DRX 600 MHz
- Bruker DRX 750 MHz
Related Database Links:
| PDB | |
| EMBL | CAA86836 |
| GB | AFM70728 EOF61613 EOH69882 EOU07149 KDR91397 |
| REF | WP_010718490 |
| SP | Q47840 |
Download simulated HSQC data in one of the following formats:
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or all simulated shifts
SPARKY: Backbone
or all simulated shifts