BMRB Entry 4367
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4367
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Backbone assignments of a Borrelia OspA mutant containing an extended single-layer beta-sheet PubMed: 10667801
Deposition date: 1999-07-14 Original release date: 2000-03-23
Authors: Huang, Xiaolin; Link, Karl; Koide, Akiko; Koide, Shohei
Citation: Koide, Shohei; Huang, Xiaolin; Link, Karl; Koide, Akiko; Bu, Zimei; Engelman, Donald. "Design of single-layer beta-sheets without a hydrophobic core" Nature 403, 456-460 (2000).
Assembly members:
OspA mutant, polymer, 280 residues, 30260 Da.
Natural source: Common Name: Lyme disease spirochete Taxonomy ID: 139 Superkingdom: not available Kingdom: not available Genus/species: Borrelia burgdorferi
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
OspA mutant: AKQNVSSLDEKNSVSVDLPG
EMKVLVSKEKNKDGKYDLIA
TVDKLELKGTSDKNNGSGVL
EGVKADKSKVKLTISDDLGQ
TTLEVFKEDGKTLVSKKVTS
KDKSSTEEKFNEKGELSEKK
ITRADKSSTEEKFNEKGEVS
EKIITRADGTRLEYTGIKSD
GSGKAKEVLKGYVLEGTLTA
EKTTLVVKEGTVTLSKNISK
SGEVSVELNDTDSSAATKKT
AAWNSGTSTLTITVNSKKTK
DLVFTKENTITVQQYDSNGT
KLEGSAVEITKLDEIKNALK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 813 |
| 15N chemical shifts | 277 |
| 1H chemical shifts | 277 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | OspA+1bh | 1 |
Entities:
Entity 1, OspA+1bh 280 residues - 30260 Da.
| 1 | ALA | LYS | GLN | ASN | VAL | SER | SER | LEU | ASP | GLU | |
| 2 | LYS | ASN | SER | VAL | SER | VAL | ASP | LEU | PRO | GLY | |
| 3 | GLU | MET | LYS | VAL | LEU | VAL | SER | LYS | GLU | LYS | |
| 4 | ASN | LYS | ASP | GLY | LYS | TYR | ASP | LEU | ILE | ALA | |
| 5 | THR | VAL | ASP | LYS | LEU | GLU | LEU | LYS | GLY | THR | |
| 6 | SER | ASP | LYS | ASN | ASN | GLY | SER | GLY | VAL | LEU | |
| 7 | GLU | GLY | VAL | LYS | ALA | ASP | LYS | SER | LYS | VAL | |
| 8 | LYS | LEU | THR | ILE | SER | ASP | ASP | LEU | GLY | GLN | |
| 9 | THR | THR | LEU | GLU | VAL | PHE | LYS | GLU | ASP | GLY | |
| 10 | LYS | THR | LEU | VAL | SER | LYS | LYS | VAL | THR | SER | |
| 11 | LYS | ASP | LYS | SER | SER | THR | GLU | GLU | LYS | PHE | |
| 12 | ASN | GLU | LYS | GLY | GLU | LEU | SER | GLU | LYS | LYS | |
| 13 | ILE | THR | ARG | ALA | ASP | LYS | SER | SER | THR | GLU | |
| 14 | GLU | LYS | PHE | ASN | GLU | LYS | GLY | GLU | VAL | SER | |
| 15 | GLU | LYS | ILE | ILE | THR | ARG | ALA | ASP | GLY | THR | |
| 16 | ARG | LEU | GLU | TYR | THR | GLY | ILE | LYS | SER | ASP | |
| 17 | GLY | SER | GLY | LYS | ALA | LYS | GLU | VAL | LEU | LYS | |
| 18 | GLY | TYR | VAL | LEU | GLU | GLY | THR | LEU | THR | ALA | |
| 19 | GLU | LYS | THR | THR | LEU | VAL | VAL | LYS | GLU | GLY | |
| 20 | THR | VAL | THR | LEU | SER | LYS | ASN | ILE | SER | LYS | |
| 21 | SER | GLY | GLU | VAL | SER | VAL | GLU | LEU | ASN | ASP | |
| 22 | THR | ASP | SER | SER | ALA | ALA | THR | LYS | LYS | THR | |
| 23 | ALA | ALA | TRP | ASN | SER | GLY | THR | SER | THR | LEU | |
| 24 | THR | ILE | THR | VAL | ASN | SER | LYS | LYS | THR | LYS | |
| 25 | ASP | LEU | VAL | PHE | THR | LYS | GLU | ASN | THR | ILE | |
| 26 | THR | VAL | GLN | GLN | TYR | ASP | SER | ASN | GLY | THR | |
| 27 | LYS | LEU | GLU | GLY | SER | ALA | VAL | GLU | ILE | THR | |
| 28 | LYS | LEU | ASP | GLU | ILE | LYS | ASN | ALA | LEU | LYS |
Samples:
sample_one: OspA mutant, [U-13C; U-15N], 1.5 mM; sodium phosphate 10 mM; sodium chloride 50 mM; EDTA 0.05 mM
sample_conditions_set_1: pH: 6.0 na; temperature: 318 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| not available | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts