BMRB Entry 4563
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4563
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Title: NMR Structure of the Bovine Prion Protein
Deposition date: 1999-12-15 Original release date: 2000-05-10
Authors: Lopez Garcia, Francisco; Zahn, Ralph; Riek, Roland; Billeter, Martin; Wuthrich, Kurt
Citation: Lopez Garcia, Francisco; Zahn, Ralph; Riek, Roland; Billeter, Martin; Wuthrich, Kurt. "NMR Structure of the Bovine Prion Protein" Proc. Natl. Acad. Sci. U.S.A. ., .-..
Assembly members:
bovine prion protein, polymer, 112 residues, 13137 Da.
Natural source: Common Name: bovine Taxonomy ID: 9913 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Bos taurus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
bovine prion protein: GSVVGGLGGYMLGSAMSRPL
IHFGSDYEDRYYRENMHRYP
NQVYYRPVDQYSNQNNFVHD
CVNITVKEHTVTTTTKGENF
TETDIKMMERVVEQMCITQY
QRESQAYYQRGA
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 789 |
| 13C chemical shifts | 356 |
| 15N chemical shifts | 129 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | bPrP(121-230) | 1 |
Entities:
Entity 1, bPrP(121-230) 112 residues - 13137 Da.
| 1 | GLY | SER | VAL | VAL | GLY | GLY | LEU | GLY | GLY | TYR | ||||
| 2 | MET | LEU | GLY | SER | ALA | MET | SER | ARG | PRO | LEU | ||||
| 3 | ILE | HIS | PHE | GLY | SER | ASP | TYR | GLU | ASP | ARG | ||||
| 4 | TYR | TYR | ARG | GLU | ASN | MET | HIS | ARG | TYR | PRO | ||||
| 5 | ASN | GLN | VAL | TYR | TYR | ARG | PRO | VAL | ASP | GLN | ||||
| 6 | TYR | SER | ASN | GLN | ASN | ASN | PHE | VAL | HIS | ASP | ||||
| 7 | CYS | VAL | ASN | ILE | THR | VAL | LYS | GLU | HIS | THR | ||||
| 8 | VAL | THR | THR | THR | THR | LYS | GLY | GLU | ASN | PHE | ||||
| 9 | THR | GLU | THR | ASP | ILE | LYS | MET | MET | GLU | ARG | ||||
| 10 | VAL | VAL | GLU | GLN | MET | CYS | ILE | THR | GLN | TYR | ||||
| 11 | GLN | ARG | GLU | SER | GLN | ALA | TYR | TYR | GLN | ARG | ||||
| 12 | GLY | ALA |
Samples:
sample_1: bovine prion protein 1.0 mM
sample_2: bovine prion protein, [U-15N], 1.0 mM
sample_3: bovine prion protein, [U-15N; U-13C], 1.0 mM
sample_conditions: pH: 4.5; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-1H-15N NOESY | not available | not available | not available |
| 1H-1H-13C NOESY | not available | not available | not available |
| HCCH COSY | not available | not available | not available |
| HCCH TOCSY | not available | not available | not available |
| HNCA | not available | not available | not available |
| HNCACB | not available | not available | not available |
Software:
PROSA -
XEASY -
DYANA -
NMR spectrometers:
- Bruker DRX 600 MHz
- Bruker DRX 750 MHz
Related Database Links:
| BMRB | 4564 |
| PDB | |
| DBJ | BAA01467 BAA01468 BAA01469 BAA19253 BAE78583 |
| EMBL | CAA39368 CAC37367 |
| GB | AAB25514 AAD19981 AAD19998 AAD19999 AAI19822 |
| REF | NP_001258555 NP_001291815 NP_851358 XP_005906596 XP_006039785 |
| SP | B5SY89 P10279 Q5UAF1 Q5UJH0 Q5UJH8 |
| TPG | DAA23426 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts