BMRB Entry 4573
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4573
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Title: 1HN,15N,13CO,13Ca,13Cb chemical shifts of 7,8-dihydroneopterin aldolase (DHNA) from Staphylococcus aureus
Deposition date: 2000-01-25 Original release date: 2004-05-15
Authors: Salzmann, Michael; Pervushin, Konstantin; Wider, Gerhard; Senn, Hans; Wuthrich, Kurt
Citation: Salzmann, Michael; Pervushin, Konstantin; Wider, Gerhard; Senn, Hans; Wuthrich, Kurt. "NMR Assignment and Secondary Structure Determination of an Octameric 110 kDa Protein Using TROSY in Triple Resonance Experiments" J. Am. Chem. Soc. 122, 7543-7548 (2000).
Assembly members:
Aldolase, polymer, 121 residues, 15000 Da.
Natural source: Common Name: Staphylococcus aureus Taxonomy ID: 1280 Superkingdom: Bacteria Kingdom: not available Genus/species: Staphylococcus aureus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Aldolase: MQDTIFLKGMRFYGYHGALS
AENEIGQIFKVDVTLKVDLS
EAGRTDNVIDTVHYGEVFEE
VKSIMEGKAVNLLEHLAERI
ANRINSQYNRVMETKVRITK
ENPPIPGHYDGVGIEIVREN
K
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 109 |
| 13C chemical shifts | 302 |
| 15N chemical shifts | 109 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | subunit-1 | 1 |
| 2 | subunit-2 | 1 |
| 3 | subunit-3 | 1 |
| 4 | subunit-4 | 1 |
| 5 | subunit-5 | 1 |
| 6 | subunit-6 | 1 |
| 7 | subunit-7 | 1 |
| 8 | subunit-8 | 1 |
Entities:
Entity 1, subunit-1 121 residues - 15000 Da.
| 1 | MET | GLN | ASP | THR | ILE | PHE | LEU | LYS | GLY | MET | ||||
| 2 | ARG | PHE | TYR | GLY | TYR | HIS | GLY | ALA | LEU | SER | ||||
| 3 | ALA | GLU | ASN | GLU | ILE | GLY | GLN | ILE | PHE | LYS | ||||
| 4 | VAL | ASP | VAL | THR | LEU | LYS | VAL | ASP | LEU | SER | ||||
| 5 | GLU | ALA | GLY | ARG | THR | ASP | ASN | VAL | ILE | ASP | ||||
| 6 | THR | VAL | HIS | TYR | GLY | GLU | VAL | PHE | GLU | GLU | ||||
| 7 | VAL | LYS | SER | ILE | MET | GLU | GLY | LYS | ALA | VAL | ||||
| 8 | ASN | LEU | LEU | GLU | HIS | LEU | ALA | GLU | ARG | ILE | ||||
| 9 | ALA | ASN | ARG | ILE | ASN | SER | GLN | TYR | ASN | ARG | ||||
| 10 | VAL | MET | GLU | THR | LYS | VAL | ARG | ILE | THR | LYS | ||||
| 11 | GLU | ASN | PRO | PRO | ILE | PRO | GLY | HIS | TYR | ASP | ||||
| 12 | GLY | VAL | GLY | ILE | GLU | ILE | VAL | ARG | GLU | ASN | ||||
| 13 | LYS |
Samples:
sample_label: Aldolase, [U-95% 2H; U-98% 13C; U-99% 15N], 0.5 mM
sample_conditions_label: pH: 6.5; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
XEASY -
NMR spectrometers:
- Bruker DRX 750 MHz
Related Database Links:
| PDB | |
| DBJ | BAB41703 BAB56677 BAB94335 BAF66749 BAF77395 |
| EMBL | CAG39538 CAG42247 CAI80152 CAQ49018 CBI48462 |
| GB | AAW37671 ABD20664 ABD29643 ABQ48341 ABR51408 |
| REF | WP_001154300 WP_001154301 WP_001154302 WP_001154303 WP_001154304 |
| SP | P56740 P64145 P64146 P64147 Q5HIG0 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts