BMRB Entry 4620
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4620
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Title: Human prion protein variant R220K
Deposition date: 2000-05-11 Original release date: 2000-12-06
Authors: Calzonai, L.; Lysek, D.; Guntert, P.; Von Schroetter, C.; Zahn, R.; Riek, R.; Wuthrich, K.
Citation: Calzonai, L.; Lysek, D.; Guntert, P.; Von Schroetter, C.; Zahn, R.; Riek, R.; Wuthrich, K.. "NMR structures of three single-residue variants of the human prion protein" Proc. Natl. Acad. Sci. U. S. A. 97, 8340-8345 (2000).
Assembly members:
prion protein, polymer, 112 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
prion protein: GSVVGGLGGYMLGSAMSRPI
IHFGSDYEDRYYRENMHRYP
NQVYYRPMDEYSNQNNFVHD
CVNITIKQHTVTTTTKGENF
TETDVKMMERVVEQMCITQY
EKESQAYYQRGS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 355 |
| 15N chemical shifts | 125 |
| 1H chemical shifts | 768 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | prion protein | 1 |
Entities:
Entity 1, prion protein 112 residues - Formula weight is not available
| 1 | GLY | SER | VAL | VAL | GLY | GLY | LEU | GLY | GLY | TYR | ||||
| 2 | MET | LEU | GLY | SER | ALA | MET | SER | ARG | PRO | ILE | ||||
| 3 | ILE | HIS | PHE | GLY | SER | ASP | TYR | GLU | ASP | ARG | ||||
| 4 | TYR | TYR | ARG | GLU | ASN | MET | HIS | ARG | TYR | PRO | ||||
| 5 | ASN | GLN | VAL | TYR | TYR | ARG | PRO | MET | ASP | GLU | ||||
| 6 | TYR | SER | ASN | GLN | ASN | ASN | PHE | VAL | HIS | ASP | ||||
| 7 | CYS | VAL | ASN | ILE | THR | ILE | LYS | GLN | HIS | THR | ||||
| 8 | VAL | THR | THR | THR | THR | LYS | GLY | GLU | ASN | PHE | ||||
| 9 | THR | GLU | THR | ASP | VAL | LYS | MET | MET | GLU | ARG | ||||
| 10 | VAL | VAL | GLU | GLN | MET | CYS | ILE | THR | GLN | TYR | ||||
| 11 | GLU | LYS | GLU | SER | GLN | ALA | TYR | TYR | GLN | ARG | ||||
| 12 | GLY | SER |
Samples:
sample_1: prion protein, [U-13C; U-15N], 1.0 mM; sodium acetate 50 mM
sample_cond_1: ionic strength: 50 mM; pH: 4.5; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCA | sample_1 | not available | sample_cond_1 |
| HN(CO)CA | sample_1 | not available | sample_cond_1 |
| HCCH-TOCSY | sample_1 | not available | sample_cond_1 |
| 15N-resolved (1H,1H)NOESY | sample_1 | not available | sample_cond_1 |
| 13C-resolved (1H,1H)NOESY | sample_1 | not available | sample_cond_1 |
Software:
No software information available
NMR spectrometers:
- Bruker DRX 750 MHz
Related Database Links:
| BMRB | 4379 4402 4434 4641 4736 5713 |
| PDB | |
| DBJ | BAA00011 BAG32276 BAG35206 BAG52189 BAG56832 |
| EMBL | CAA58442 CAB75503 CAM27320 |
| GenBank | AAA19664 AAA60182 AAA68633 AAB59442 AAB59443 |
| REF | NP_000302 NP_001073590 NP_001073591 NP_001073592 NP_898902 |
| SWISS-PROT | P04156 P40252 |
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