BMRB Entry 4770
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4770
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Backbone 1HN, 15N and 13C shifts for GDP-loaded Cdc42 from Candida albicans
Deposition date: 2000-06-27 Original release date: 2003-06-12
Authors: Osborne, Michael; Stevens, Willem; Ping, Xu; Ni, Feng
Citation: Osborne, Michael; Su, Zhengding; Sridaran, Vasanth; Ni, Feng. "Efficient expression of isotopically labeled peptides for high resolution NMR studies: Application to the Cdc42/Rac binding domains of virulent kinases in Candida albicans " J. Biomol. NMR 26, 317-326 (2003).
Assembly members:
Cell division control protein 42, polymer, 181 residues, Formula weight is not available
GUANOSINE-5'-DIPHOSPHATE, non-polymer, 443.201 Da.
Natural source: Common Name: Yeast Taxonomy ID: 5476 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Candida albicans
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Cell division control protein 42: GSHMQTIKCVVVGDGAVGKT
CLLISYTTSKFPADYVPTVF
DNYAVTVMIGDEPFTLGLFD
TAGQEDYDRLRPLSYPSTDV
FLVCFSVISPASFENVKEKW
FPEVHHHCPGVPIIIVGTQT
DLRNDDVILQRLHRQKLSPI
TQEQGEKLAKELRAVKYVEC
SALTQRGLKTVFDEAIVAAL
E
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 138 |
| 13C chemical shifts | 258 |
| 15N chemical shifts | 138 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Cdc42 | 1 |
Entities:
Entity 1, Cdc42 181 residues - Formula weight is not available
| 1 | GLY | SER | HIS | MET | GLN | THR | ILE | LYS | CYS | VAL | ||||
| 2 | VAL | VAL | GLY | ASP | GLY | ALA | VAL | GLY | LYS | THR | ||||
| 3 | CYS | LEU | LEU | ILE | SER | TYR | THR | THR | SER | LYS | ||||
| 4 | PHE | PRO | ALA | ASP | TYR | VAL | PRO | THR | VAL | PHE | ||||
| 5 | ASP | ASN | TYR | ALA | VAL | THR | VAL | MET | ILE | GLY | ||||
| 6 | ASP | GLU | PRO | PHE | THR | LEU | GLY | LEU | PHE | ASP | ||||
| 7 | THR | ALA | GLY | GLN | GLU | ASP | TYR | ASP | ARG | LEU | ||||
| 8 | ARG | PRO | LEU | SER | TYR | PRO | SER | THR | ASP | VAL | ||||
| 9 | PHE | LEU | VAL | CYS | PHE | SER | VAL | ILE | SER | PRO | ||||
| 10 | ALA | SER | PHE | GLU | ASN | VAL | LYS | GLU | LYS | TRP | ||||
| 11 | PHE | PRO | GLU | VAL | HIS | HIS | HIS | CYS | PRO | GLY | ||||
| 12 | VAL | PRO | ILE | ILE | ILE | VAL | GLY | THR | GLN | THR | ||||
| 13 | ASP | LEU | ARG | ASN | ASP | ASP | VAL | ILE | LEU | GLN | ||||
| 14 | ARG | LEU | HIS | ARG | GLN | LYS | LEU | SER | PRO | ILE | ||||
| 15 | THR | GLN | GLU | GLN | GLY | GLU | LYS | LEU | ALA | LYS | ||||
| 16 | GLU | LEU | ARG | ALA | VAL | LYS | TYR | VAL | GLU | CYS | ||||
| 17 | SER | ALA | LEU | THR | GLN | ARG | GLY | LEU | LYS | THR | ||||
| 18 | VAL | PHE | ASP | GLU | ALA | ILE | VAL | ALA | ALA | LEU | ||||
| 19 | GLU |
Samples:
sample_1: Cell division control protein 42, [U-15N; U-13C], 0.8 mM; GUANOSINE-5'-DIPHOSPHATE 6 mM; sodium phosphate 50 mM; magnesium chloride 2 mM; sodium chloride 50 mM
cond_1: pH*: 6.8; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCA | not available | not available | not available |
| HN(CO)CA | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| HNCACB | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker DRX 800 MHz
Related Database Links:
| BMRB | 4778 |
| EMBL | CAX45095 CCE43086 CCG21049 |
| GB | AAB69764 EAK92670 EAK92699 EDK42478 EEQ42372 |
| REF | XP_001528136 XP_002417442 XP_002548861 XP_003866489 XP_007376934 |
| SP | C4YDI6 P0CY33 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts