BMRB Entry 4791
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4791
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Title: Sequence-specific 1H, 15N and 13C Resonance Assignments for an Engineered Arginine-rich Domain of the Hepatitis C Virus NS3 RNA Helicase
Deposition date: 2000-07-21 Original release date: 2001-05-03
Authors: Liu, Dingjiang; Wyss, Daniel
Citation: Liu, Dingjiang; Wyss, Daniel. "Letter to the Editor: Sequence-specific 1H, 15N and 13C Resonance Assignments for an Engineered Arginine-rich Domain of the Hepatitis C Virus NS3 RNA Helicase" J. Biomol. NMR 18, 279-280 (2000).
Assembly members:
HCV helicase, polymer, 142 residues, Formula weight is not available
Natural source: Common Name: hepatitis C virus Taxonomy ID: 11103 Superkingdom: viruses Kingdom: not available Genus/species: hepatitis C virus hepatitis C virus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
HCV helicase: GSHMGSVTVPHPNIEEVALS
TTGEIPFYGKAIPLEVIKGG
RHLIFCHSKKKCDELAAKLV
ALGINAVAYYRGLDVSVIPT
NGDVVVVATDALMTGFTGDF
DSVIDCNTSDGKPQDAVSRT
QRRGRTGRGKPGIYRFVAPG
ER
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 577 |
| 15N chemical shifts | 143 |
| 1H chemical shifts | 942 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HCV NS3 RNA helicase domain 2 | 1 |
Entities:
Entity 1, HCV NS3 RNA helicase domain 2 142 residues - Formula weight is not available
| 1 | GLY | SER | HIS | MET | GLY | SER | VAL | THR | VAL | PRO | ||||
| 2 | HIS | PRO | ASN | ILE | GLU | GLU | VAL | ALA | LEU | SER | ||||
| 3 | THR | THR | GLY | GLU | ILE | PRO | PHE | TYR | GLY | LYS | ||||
| 4 | ALA | ILE | PRO | LEU | GLU | VAL | ILE | LYS | GLY | GLY | ||||
| 5 | ARG | HIS | LEU | ILE | PHE | CYS | HIS | SER | LYS | LYS | ||||
| 6 | LYS | CYS | ASP | GLU | LEU | ALA | ALA | LYS | LEU | VAL | ||||
| 7 | ALA | LEU | GLY | ILE | ASN | ALA | VAL | ALA | TYR | TYR | ||||
| 8 | ARG | GLY | LEU | ASP | VAL | SER | VAL | ILE | PRO | THR | ||||
| 9 | ASN | GLY | ASP | VAL | VAL | VAL | VAL | ALA | THR | ASP | ||||
| 10 | ALA | LEU | MET | THR | GLY | PHE | THR | GLY | ASP | PHE | ||||
| 11 | ASP | SER | VAL | ILE | ASP | CYS | ASN | THR | SER | ASP | ||||
| 12 | GLY | LYS | PRO | GLN | ASP | ALA | VAL | SER | ARG | THR | ||||
| 13 | GLN | ARG | ARG | GLY | ARG | THR | GLY | ARG | GLY | LYS | ||||
| 14 | PRO | GLY | ILE | TYR | ARG | PHE | VAL | ALA | PRO | GLY | ||||
| 15 | GLU | ARG |
Samples:
sample-1: HCV helicase, [U-15N; U-13C], 0.2 – 1.0 mM; KPO4 75 mM; DTT, [U-2H], 5 mM; D2O 99%; AEBSF 0.4 mM; NaN3 0.015%
sample-2: HCV helicase, [U-15N; U-13C], 0.2 – 1.0 mM; KPO4 75 mM; DTT, [U-2H], 5 mM; D2O 5%; AEBSF 0.4 mM; NaN3 0.015%
cond_set_1: pH: 6.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| HNCACB | not available | not available | not available |
| HNCO | not available | not available | not available |
| (H)CC(CO)NH-TOCSY | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
| 15N-edited TOCSY-HSQC | not available | not available | not available |
Software:
FELIX v98.0 - data processing
NMRView v4.0.3 - data analysis
NMR spectrometers:
- Varian INOVA 500 MHz
- Varian INOVA 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts