BMRB Entry 4816

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4816

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Structural Features of an Influenza Virus Promoter and their Implications for Viral RNA Synthesis PubMed: 11553808

Deposition date: 2000-08-23 Original release date: 2002-04-04

Authors: Bae, S.-H.; Cheong, H.-K.; Lee, J.-H.; Cheong, C.; Kainosho, M.; Choi, B.-S.

Citation: Bae, S.-H.; Cheong, H.-K.; Lee, J.-H.; Cheong, C.; Kainosho, M.; Choi, B.-S.. "Structural Features of an Influenza Virus Promoter and Their Implications for Viral RNA Synthesis" Proc. Natl. Acad. Sci. U.S.A. 98, 10602-10607 (2001).

Assembly members:
INFLUENZA A VIRUS PROMOTER RNA, polymer, 31 residues, Formula weight is not available

Natural source: Common Name: not available Taxonomy ID: 11320 Superkingdom: viruses Kingdom: not available Genus/species: Influenza virus type A Influenza A virus

Experimental source: Production method: enzymatic semisynthesis

Entity Sequences (FASTA):
INFLUENZA A VIRUS PROMOTER RNA: AGUAGAAACAAGGCUUCGGC CUGCUUUUGCU

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
13C chemical shifts	39
15N chemical shifts	23
31P chemical shifts	22

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	VRNA	1

Entities:

Entity 1, VRNA 31 residues - Formula weight is not available

1

A

G

U

A

G

A

C

A

2

A

G

C

U

C

G

C

3

C

U

G

C

U

G

C

4

U

Samples:

vRNA-H2O: INFLUENZA A VIRUS PROMOTER RNA 2 mM; Na phosphate 10 mM; EDTA 0.1 mM; H2O 90%; D2O 10%

vRNA-D2O: INFLUENZA A VIRUS PROMOTER RNA 2 mM; Na phosphate 10 mM; EDTA 0.1 mM; D2O 99.96%

vRNA-CN-H2O: INFLUENZA A VIRUS PROMOTER RNA, [U-13C; U-15N], 0.4 mM; Na phosphate 10 mM; EDTA 0.1 mM; H2O 90%; D2O 10%

vRNA-CN-D2O: INFLUENZA A VIRUS PROMOTER RNA, [U-13C; U-15N], 0.4 mM; Na phosphate 10 mM; EDTA 0.1 mM; D2O 99.96%

conditions_1: ionic strength: 10 mM; pH: 6.5; temperature: 272 K

conditions_2: ionic strength: 10 mM; pH: 6.5; temperature: 278 K

conditions_3: ionic strength: 10 mM; pH: 6.5; temperature: 294 K

vRNA-conditions_4: ionic strength: 10 mM; pH: 6.5; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D NOESY	not available	not available	not available
DQF-COSY	not available	not available	not available
31P-1H HETCOR	not available	not available	not available

Software:

xwinnmr v2.6 - collection, processing

SPARKY v3.87 - data analysis

X-PLOR v3.1 - refinement

NMR spectrometers:

Bruker DRX 400 MHz