BMRB Entry 4840
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4840
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Title: 1H, 13C and 15N resonance assignment and secondary structure of Mycobacterium tuberculosis adenylate kinase
Deposition date: 2000-09-26 Original release date: 2001-02-14
Authors: Miron, Simona; Munier-Lehmann, Helene; Craescu, Constantin
Citation: Miron, Simona; Munier-Lehmann, Helene; Craescu, Constantin. "Letter to the Editor: 1H, 13C and 15N resonance assignment and secondary structure of Mycobacterium tuberculosis adenylate kinase" J. Biomol. NMR 19, 89-90 (2001).
Assembly members:
Adenylate kinase from M. tuberculosis, polymer, 181 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: 1773 Superkingdom: Bacteria Kingdom: not available Genus/species: Mycobacterium tuberculosis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Adenylate kinase from M. tuberculosis: MRVLLLGPPGAGKGTQAVKL
AEKLGIPQISTGELFRRNIE
EGTKLGVEAKRYLDAGDLVP
SDLTNELVDDRLNNPDAANG
FILDGYPRSVEQAKALHEML
ERRGTDIDAVLEFRVSEEVL
LERLKGRGRADDTDDVILNR
MKVYRDETAPLLEYYRDQLK
TVDAVGTMDEVFARALRALG
K
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 848 |
| 13C chemical shifts | 333 |
| 15N chemical shifts | 178 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | AK | 1 |
Entities:
Entity 1, AK 181 residues - Formula weight is not available
| 1 | MET | ARG | VAL | LEU | LEU | LEU | GLY | PRO | PRO | GLY | ||||
| 2 | ALA | GLY | LYS | GLY | THR | GLN | ALA | VAL | LYS | LEU | ||||
| 3 | ALA | GLU | LYS | LEU | GLY | ILE | PRO | GLN | ILE | SER | ||||
| 4 | THR | GLY | GLU | LEU | PHE | ARG | ARG | ASN | ILE | GLU | ||||
| 5 | GLU | GLY | THR | LYS | LEU | GLY | VAL | GLU | ALA | LYS | ||||
| 6 | ARG | TYR | LEU | ASP | ALA | GLY | ASP | LEU | VAL | PRO | ||||
| 7 | SER | ASP | LEU | THR | ASN | GLU | LEU | VAL | ASP | ASP | ||||
| 8 | ARG | LEU | ASN | ASN | PRO | ASP | ALA | ALA | ASN | GLY | ||||
| 9 | PHE | ILE | LEU | ASP | GLY | TYR | PRO | ARG | SER | VAL | ||||
| 10 | GLU | GLN | ALA | LYS | ALA | LEU | HIS | GLU | MET | LEU | ||||
| 11 | GLU | ARG | ARG | GLY | THR | ASP | ILE | ASP | ALA | VAL | ||||
| 12 | LEU | GLU | PHE | ARG | VAL | SER | GLU | GLU | VAL | LEU | ||||
| 13 | LEU | GLU | ARG | LEU | LYS | GLY | ARG | GLY | ARG | ALA | ||||
| 14 | ASP | ASP | THR | ASP | ASP | VAL | ILE | LEU | ASN | ARG | ||||
| 15 | MET | LYS | VAL | TYR | ARG | ASP | GLU | THR | ALA | PRO | ||||
| 16 | LEU | LEU | GLU | TYR | TYR | ARG | ASP | GLN | LEU | LYS | ||||
| 17 | THR | VAL | ASP | ALA | VAL | GLY | THR | MET | ASP | GLU | ||||
| 18 | VAL | PHE | ALA | ARG | ALA | LEU | ARG | ALA | LEU | GLY | ||||
| 19 | LYS |
Samples:
sample_one: Adenylate kinase from M. tuberculosis 1.0 mM; H2O 95%; D2O 5%
sample_two: Adenylate kinase from M. tuberculosis, [U-15N], 1.0 mM; H2O 95%; D2O 5%
sample_three: Adenylate kinase from M. tuberculosis, [U-15N; U-13C], 1.2 mM; H2O 95%; D2O 5%
sample_conditions_one: pH: 7.1; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
No software information available
NMR spectrometers:
- Varian Unity 500 MHz
- Varian Inova 750 MHz
Related Database Links:
| PDB | |
| DBJ | BAH25046 BAL64618 BAQ04633 GAA44509 |
| EMBL | CAD93616 CAL70769 CCC25815 CCC43078 CCC63341 |
| GB | AAD09879 AAK44991 ABQ72469 ABR05090 ACT23783 |
| REF | NP_215247 NP_335177 NP_854412 WP_003403726 WP_003911271 |
| SP | A1KGL4 A5U0B9 C1AL67 P69439 P9WKF4 |
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