BMRB Entry 4885
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4885
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Title: Backbone 1H, 15N and 13C Resonance Assignments of the NTPase Subdomain of the Hepatitis C Virus NS3 RNA Helicase PubMed: 11330818
Deposition date: 2000-10-25 Original release date: 2001-11-14
Authors: Liu, Dingjiang; Wyss, Daniel
Citation: Liu, Dingjiang; Wyss, Daniel. "Backbone 1H, 15N and 13C Resonance Assignments of the NTPase Subdomain of the Hepatitis C Virus NS3 RNA Helicase" J. Biomol. NMR 19, 283-284 (2001).
Assembly members:
HCV helicase, polymer, 144 residues, Formula weight is not available
Natural source: Common Name: hepatitis C virus Taxonomy ID: 31646 Superkingdom: viruses Kingdom: not available Genus/species: viruses hepatitis C virus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
HCV helicase: SPVFTDNSSPPAVPQSFQVA
HLHAPTGSGKSTKVPAAYAA
QGYKVLVLNPSVAATLGFGA
YMSKAHGVDPNIRTGVRTIT
TGSPITYSTYGKFLADGGCS
GGAYDIIICDECHSTDATSI
LGIGTVLDQAETAGARLVVL
ATAT
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 119 |
| 13C chemical shifts | 356 |
| 15N chemical shifts | 119 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HCV helicase NTPase domain | 1 |
Entities:
Entity 1, HCV helicase NTPase domain 144 residues - Formula weight is not available
| 1 | SER | PRO | VAL | PHE | THR | ASP | ASN | SER | SER | PRO | ||||
| 2 | PRO | ALA | VAL | PRO | GLN | SER | PHE | GLN | VAL | ALA | ||||
| 3 | HIS | LEU | HIS | ALA | PRO | THR | GLY | SER | GLY | LYS | ||||
| 4 | SER | THR | LYS | VAL | PRO | ALA | ALA | TYR | ALA | ALA | ||||
| 5 | GLN | GLY | TYR | LYS | VAL | LEU | VAL | LEU | ASN | PRO | ||||
| 6 | SER | VAL | ALA | ALA | THR | LEU | GLY | PHE | GLY | ALA | ||||
| 7 | TYR | MET | SER | LYS | ALA | HIS | GLY | VAL | ASP | PRO | ||||
| 8 | ASN | ILE | ARG | THR | GLY | VAL | ARG | THR | ILE | THR | ||||
| 9 | THR | GLY | SER | PRO | ILE | THR | TYR | SER | THR | TYR | ||||
| 10 | GLY | LYS | PHE | LEU | ALA | ASP | GLY | GLY | CYS | SER | ||||
| 11 | GLY | GLY | ALA | TYR | ASP | ILE | ILE | ILE | CYS | ASP | ||||
| 12 | GLU | CYS | HIS | SER | THR | ASP | ALA | THR | SER | ILE | ||||
| 13 | LEU | GLY | ILE | GLY | THR | VAL | LEU | ASP | GLN | ALA | ||||
| 14 | GLU | THR | ALA | GLY | ALA | ARG | LEU | VAL | VAL | LEU | ||||
| 15 | ALA | THR | ALA | THR |
Samples:
sample_1: HCV helicase, [U-15N; U-13C; U-2H], 0.075 – 0.5 mM
condition_1: pH: 7.4; temperature: 293 K; ionic strength: 0.1 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N NOESY-HSQC | not available | not available | not available |
| 1H-15N TOCSY-HSQC | not available | not available | not available |
| HNCA | not available | not available | not available |
| HNCOCA | not available | not available | not available |
| HNCOCACB | not available | not available | not available |
| HNCACB | not available | not available | not available |
| HNCO | not available | not available | not available |
Software:
FELIX v98 -
NMRView v4.1.2 -
NMR spectrometers:
- Varian UNITYplus 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAA01582 BAA03178 BAA08120 BAA09073 BAA09075 |
| EMBL | CAC03609 CAJ20150 CAJ20155 CAJ20158 CAJ20165 |
| GB | AAA45534 AAA45676 AAA45677 AAB02124 AAB02125 |
| REF | NP_671491 NP_803144 |
| SP | P26664 P27958 Q03463 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts