BMRB Entry 4928
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4928
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Title: Structure of the PHD Zinc finger from human Williams-Beuren syndrome transcription factor
Deposition date: 2000-12-18 Original release date: 2001-01-19
Authors: Pascual, Jaime; Martinez-Yamout, M.; Dyson, H. Jane; Wright, Peter
Citation: Pascual, Jaime; Martinez-Yamout, M.; Dyson, H. Jane; Wright, Peter. "Structure of the PHD Zinc finger from human Williams-Beuren syndrome transcription factor" J. Mol. Biol. 304, 723-729 (2000).
Assembly members:
WSTF-PHD, polymer, 51 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
WSTF-PHD: ARCKVCRKKGEDDKLILCDE
CNKAFHLFCLRPALYEVPDG
EWQCPACQPAT
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 340 |
| 13C chemical shifts | 202 |
| 15N chemical shifts | 49 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | wstf-phd | 1 |
| 2 | Zn-1 | 2 |
| 3 | Zn-2 | 2 |
Entities:
Entity 1, wstf-phd 51 residues - Formula weight is not available
| 1 | ALA | ARG | CYS | LYS | VAL | CYS | ARG | LYS | LYS | GLY | ||||
| 2 | GLU | ASP | ASP | LYS | LEU | ILE | LEU | CYS | ASP | GLU | ||||
| 3 | CYS | ASN | LYS | ALA | PHE | HIS | LEU | PHE | CYS | LEU | ||||
| 4 | ARG | PRO | ALA | LEU | TYR | GLU | VAL | PRO | ASP | GLY | ||||
| 5 | GLU | TRP | GLN | CYS | PRO | ALA | CYS | GLN | PRO | ALA | ||||
| 6 | THR |
Entity 2, Zn-1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample: WSTF-PHD, [U-15N; U-13C], 1.0 mM
sample_conditions: pH: 6.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCO | sample | not available | sample_conditions |
| HNCACB | sample | not available | sample_conditions |
| CBCA(CO)NH | sample | not available | sample_conditions |
| H(C)CH-COSY | sample | not available | sample_conditions |
| H(CCO)NH-TOCSY (mixing-time, tmix= 16.5 ms) | sample | not available | sample_conditions |
| C(CO)NH-TOCSY (tmix= 16.5 ms) | sample | not available | sample_conditions |
| 15N HSQC-TOCSY (tmix= 75 ms) | sample | not available | sample_conditions |
| 15N HSQC-NOESY (tmix= 80 ms) | sample | not available | sample_conditions |
| 13C HSQC-NOESY (tmix= 80 ms) | sample | not available | sample_conditions |
Software:
No software information available
NMR spectrometers:
- Bruker AMX 500 MHz
- Bruker DMX 750 MHz
Related Database Links:
| SWISS-PROT | Q9Z277 Q9UIG0 |
| REF | XP_001111145 XP_001111105 XP_001077467 NP_115784 NP_035844 |
| GenBank | EAW69679 AAD08676 AAD08675 AAD04720 AAC97879 |
| DBJ | BAC29862 BAA89210 |
| PDB |
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