BMRB Entry 4951
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Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4951
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Title: Structure of the Anchor-Domain of Myristoylated and Non-myristoylated HIV-1 Nef Protein
Deposition date: 2001-01-31 Original release date: 2001-03-09
Authors: Geyer, Matthias; Munte, Claudia; Kalbitzer, Hans
Citation: Geyer, Matthias; Munte, Claudia; Schorr, Jacqueline; Kellner, Roland; Kalbitzer, Hans. "Structure of the Anchor-Domain of Myristoylated and Non-myristoylated HIV-1 Nef Protein" J. Mol. Biol. 289, 123-138 (1999).
Assembly members:
HIV-1 Negative Factor, polymer, 58 residues, 6022 Da.
Natural source: Common Name: Human immunodeficiency virus type 1 Taxonomy ID: 11676 Superkingdom: viruses Kingdom: not available Genus/species: HIV-1 BEN allele (Nef_Hv112)
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
HIV-1 Negative Factor: XGGKWSKSSVVGWPAVRERM
RRAEPAADGVGAASRDLEKH
GAITSSNTAANNAACAWX
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 410 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | myristoylated Nef anchor domain (Myr-2-57) | 1 |
Entities:
Entity 1, myristoylated Nef anchor domain (Myr-2-57) 58 residues - 6022 Da.
| 1 | MYR | GLY | GLY | LYS | TRP | SER | LYS | SER | SER | VAL | ||||
| 2 | VAL | GLY | TRP | PRO | ALA | VAL | ARG | GLU | ARG | MET | ||||
| 3 | ARG | ARG | ALA | GLU | PRO | ALA | ALA | ASP | GLY | VAL | ||||
| 4 | GLY | ALA | ALA | SER | ARG | ASP | LEU | GLU | LYS | HIS | ||||
| 5 | GLY | ALA | ILE | THR | SER | SER | ASN | THR | ALA | ALA | ||||
| 6 | ASN | ASN | ALA | ALA | CYS | ALA | TRP | NH2 |
Samples:
sample_1: HIV-1 Negative Factor 0.37 mM
Ex-cond_1: pH: 4.6; temperature: 285 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-1H NOESY | sample_1 | not available | Ex-cond_1 |
| ROESY | sample_1 | not available | Ex-cond_1 |
| TOCSY | sample_1 | not available | Ex-cond_1 |
| COSY | sample_1 | not available | Ex-cond_1 |
Software:
AURELIA - automated peak assignments
NMR spectrometers:
- Bruker DMX Avance 800 MHz
- Bruker AMX 500 MHz
Related Database Links:
| BMRB | 4950 |
| PDB | |
| DBJ | BAC02670.1 BAC02671.1 |
| EMBL | CAA26947.1 |
| GenBank | AAA44206.1 AAA45001.1 AAB59874.1 AAD02461.1 |
| PIR | S03245 |
| PRF | 1103299A |
| SWISS-PROT | P04324 P05854 P03404 |