BMRB Entry 5197
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5197
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Title: 1H and 13CA chemical shift assignments of the polypeptide mPrP(143-158) PubMed: 10397798
Deposition date: 2001-10-29 Original release date: 2001-11-12
Authors: Liu, Aizhuo; Riek, Roland; Zahn, Ralph; Glockshuber, Rudolf; Wuthrich, Kurt
Citation: Liu, Aizhuo; Riek, Roland; Zahn, Ralph; Glockshuber, Rudolf; Wuthrich, Kurt. "Helix protensity of a polypeptide containing helix 1 of the mouse prion protein studied by NMR and CD spectroscopy " Biopolymers 51, 145-152 (1999).
Assembly members:
mPrP(143-158), polymer, 16 residues, Formula weight is not available
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
mPrP(143-158): NDWEDRYYRENMYRYP
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 604 |
| 13C chemical shifts | 64 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | mouse prion protein, trans conformer | 1 |
| 2 | mouse prion protein, cis conformer | 1 |
Entities:
Entity 1, mouse prion protein, trans conformer 16 residues - Formula weight is not available
| 1 | ASN | ASP | TRP | GLU | ASP | ARG | TYR | TYR | ARG | GLU | ||||
| 2 | ASN | MET | TYR | ARG | TYR | PRO |
Samples:
sample_1: mPrP(143-158) 1.0 mM; H2O 90%; D2O 10%
sample_2: mPrP(143-158) 1.0 mM; H2O 90%; TFE, [U-2H], 10%
sample_3: mPrP(143-158) 1.0 mM; H2O 75%; TFE, [U-2H], 25%
sample_4: mPrP(143-158) 1.0 mM; H2O 50%; TFE, [U-2H], 50%
Ex-cond_1: pH: 4.5; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| not available | not available | not available | Ex-cond_1 |
Software:
No software information available
NMR spectrometers:
- Varian INOVA 400 MHz
- Bruker DRX 500 MHz
Related Database Links:
| BMRB | 15845 16071 16075 16076 16077 16078 16079 16080 16184 16185 16722 16723 17081 17082 17084 17087 17174 17213 17758 17759 |
| PDB | |
| DBJ | BAA08790 BAE28320 BAE28693 BAE29994 BAE34221 |
| EMBL | CAJ18553 |
| GB | AAA39996 AAA39997 AAA39998 AAA39999 AAA41947 |
| PRF | 1106228A |
| REF | NP_001265185 NP_035300 NP_036763 XP_006235124 |
| SP | P04925 P13852 Q9Z0T3 |