BMRB Entry 5353
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5353
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Title: 1H, 15N and 13C resonance assignments of yeast Saccharomyces cerevisiae calmodulin in the Ca2+-free state
Deposition date: 2002-04-25 Original release date: 2002-09-25
Authors: Ishida, Hiroaki
Citation: Ishida, Hiroaki; Nakashima, K-i; Kumaki, Yasuhiro; Nakata, Mitsuo; Hikichi, Kunio; Yazawa, Michio. "Letter to the Editor: 1H, 15N and 13C resonance assignments of yeast Saccharomyces cerevisiae calmodulin in the Ca2+-free state" J. Biomol. NMR 23, 323-324 (2002).
Assembly members:
yeast calmodulin, polymer, 146 residues, 16000 Da.
Natural source: Common Name: Baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota Fungi
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
yeast calmodulin: SSNLTEEQIAEFKEAFALFD
KDNNGSISSSELATVMRSLG
LSPSEAEVNDLMNEIDVDGN
HQIEFSEFLALMSRQLKSND
SEQELLEAFKVFDKNGDGLI
SAAELKHVLTSIGEKLTDAE
VDDMLREVSDGSGEINIQQF
AALLSK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 142 |
| 15N chemical shifts | 141 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | yCaM monomer | 1 |
Entities:
Entity 1, yCaM monomer 146 residues - 16000 Da.
| 1 | SER | SER | ASN | LEU | THR | GLU | GLU | GLN | ILE | ALA | ||||
| 2 | GLU | PHE | LYS | GLU | ALA | PHE | ALA | LEU | PHE | ASP | ||||
| 3 | LYS | ASP | ASN | ASN | GLY | SER | ILE | SER | SER | SER | ||||
| 4 | GLU | LEU | ALA | THR | VAL | MET | ARG | SER | LEU | GLY | ||||
| 5 | LEU | SER | PRO | SER | GLU | ALA | GLU | VAL | ASN | ASP | ||||
| 6 | LEU | MET | ASN | GLU | ILE | ASP | VAL | ASP | GLY | ASN | ||||
| 7 | HIS | GLN | ILE | GLU | PHE | SER | GLU | PHE | LEU | ALA | ||||
| 8 | LEU | MET | SER | ARG | GLN | LEU | LYS | SER | ASN | ASP | ||||
| 9 | SER | GLU | GLN | GLU | LEU | LEU | GLU | ALA | PHE | LYS | ||||
| 10 | VAL | PHE | ASP | LYS | ASN | GLY | ASP | GLY | LEU | ILE | ||||
| 11 | SER | ALA | ALA | GLU | LEU | LYS | HIS | VAL | LEU | THR | ||||
| 12 | SER | ILE | GLY | GLU | LYS | LEU | THR | ASP | ALA | GLU | ||||
| 13 | VAL | ASP | ASP | MET | LEU | ARG | GLU | VAL | SER | ASP | ||||
| 14 | GLY | SER | GLY | GLU | ILE | ASN | ILE | GLN | GLN | PHE | ||||
| 15 | ALA | ALA | LEU | LEU | SER | LYS |
Samples:
sample_1: yeast calmodulin, [U-13C; U-15N], 1.0 mM; yeast calmodulin, [U-13C; U-15N], 1.4 mM
sample_2: yeast calmodulin, [U-15N], 1.0 mM; yeast calmodulin, [U-15N], 1.4 mM
experimental_conditions: pH: 7.0; temperature: 303 K; ionic strength: 0.05 M; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HSQC | not available | not available | not available |
| HNCA | not available | not available | not available |
| HN(CO)CA | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| HNHA | not available | not available | not available |
| HNCO | not available | not available | not available |
| HBHA(CBCACO)NH | not available | not available | not available |
| H(CCO)NH | not available | not available | not available |
| C(CO)NH | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
Software:
XEASY - peak assignments
NMR spectrometers:
- Varian UNITY 500 MHz
- JEOL JNM 600 MHz
Related Database Links:
| BMRB | 17850 |
| PDB | |
| DBJ | GAA21657 |
| EMBL | CAA55612 CAA85064 CAG58389 CAR27417 CBK39182 |
| GB | AAA34504 AAC68888 AAS56510 AHY74589 EDN64723 |
| REF | NP_009667 XP_001642859 XP_002496350 XP_003678123 XP_003680602 |
| SP | P06787 |
| TPG | DAA07228 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts