BMRB Entry 5500
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5500
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Title: Parkin binds the Rpn10 subunit of 26S proteasomes with the ubiquitin-like domain
Deposition date: 2002-08-13 Original release date: 2003-03-10
Authors: Sakata, Eri; Yamaguchi, Yoshiki; Kurimoto, Eiji; Kikuchi, Jun; Yokoyama, Shigeyuki; Kawahara, Hiroyuki; Yokosawa, Hideyoshi; Hattori, Nobutaka; Mizuno, Yoshikuni; Tanaka, Keiji; Kato, Koichi
Citation: Sakata, Eri; Yamaguchi, Yoshiki; Kurimoto, Eiji; Kikuchi, Jun; Yokoyama, Shigeyuki; Kawahara, Hiroyuki; Yokosawa, Hideyoshi; Hattori, Nobutaka; Mizuno, Yoshikuni; Tanaka, Keiji; Kato, Koichi. "Parkin binds the Rpn10 subunit of 26S proteasomes with the ubiquitin-like domain" EMBO Rep. 4, 301-306 (2003).
Assembly members:
Parkin ubiquitin-like domain, polymer, 81 residues, 9235 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Parkin ubiquitin-like domain: GPLGSMIVFVRFNSSHGFPV
EVDSDTSIFQLKEVVAKRQG
VPADQLRVIFAGKELRNDWT
VQNCDLDQQSIVHIVQRPWR
K
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 148 |
| 13C chemical shifts | 216 |
| 15N chemical shifts | 72 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Ubl | 1 |
Entities:
Entity 1, Ubl 81 residues - 9235 Da.
| 1 | GLY | PRO | LEU | GLY | SER | MET | ILE | VAL | PHE | VAL | ||||
| 2 | ARG | PHE | ASN | SER | SER | HIS | GLY | PHE | PRO | VAL | ||||
| 3 | GLU | VAL | ASP | SER | ASP | THR | SER | ILE | PHE | GLN | ||||
| 4 | LEU | LYS | GLU | VAL | VAL | ALA | LYS | ARG | GLN | GLY | ||||
| 5 | VAL | PRO | ALA | ASP | GLN | LEU | ARG | VAL | ILE | PHE | ||||
| 6 | ALA | GLY | LYS | GLU | LEU | ARG | ASN | ASP | TRP | THR | ||||
| 7 | VAL | GLN | ASN | CYS | ASP | LEU | ASP | GLN | GLN | SER | ||||
| 8 | ILE | VAL | HIS | ILE | VAL | GLN | ARG | PRO | TRP | ARG | ||||
| 9 | LYS |
Samples:
sample_1: Parkin ubiquitin-like domain, [U-98% 13C; U-98% 15N], 0.1 mM; potasium phosphate buffer 50 mM
Ex-cond_1: pH: 6.0; temperature: 303 K; ionic strength: 0.3 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | not available | not available | not available |
| 2D 1H-13C HSQC | not available | not available | not available |
| 3D CBCA(CO)NH | not available | not available | not available |
| 3D CBCANH | not available | not available | not available |
| 3D HCACO | not available | not available | not available |
| 3D HNCA | not available | not available | not available |
| 3D HN(CO)CA | not available | not available | not available |
| 3D HNCO | not available | not available | not available |
| 3D 15N-edited TOCSY | not available | not available | not available |
Software:
XWINNMR v2.6 - raw spectral data processing, data analysis
CNS v1.1 - solution structure refinement
NMR spectrometers:
- Bruker DRX 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAA25751 BAF43729 BAF85279 BAI46122 |
| EMBL | CAH90914 |
| GB | AAH22014 ABC74794 ABN46990 ACL68652 ACL98151 |
| REF | NP_001125521 NP_001274591 NP_004553 NP_054642 NP_054643 |
| SP | O60260 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts