BMRB Entry 5701
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5701
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for the catalytic GEF domain of Salmonella SopE2 PubMed: 12815267
Deposition date: 2003-02-18 Original release date: 2003-10-17
Authors: Williams, Christopher
Citation: Williams, Christopher; Galyov, Edoudard; Bagby, Stefan. "Letter to the Editor: Assignment of 1H, 13C and 15N Resonances of the Catalytic Domain of Guanine Nucleotide Exchange Factor SopE2 from Salmonella dublin" J. Biomol. NMR 26, 379-380 (2003).
Assembly members:
Salmonella SopE2, polymer, 172 residues, 18398.1 Da.
Natural source: Common Name: Salmonella Taxonomy ID: 28901 Superkingdom: Eubacteria Kingdom: not available Genus/species: Salmonella enterica subsp enterica ser typhimurium
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Salmonella SopE2: GNASEGRAVLTSKTVKDFML
QKLNSLDIKGNASKDPAYAR
QTCEAILSAVYSNNKDQCCK
LLISKGVSITPFLKEIGEAA
QNAGLPGEIKNGVFTPGGAG
ANPFVVPLIASASIKYPHMF
INHNQQVSFKAYAEKIVMKE
VTPLFNKGTMPTPQQFQLTI
ENIANKYLQNAS
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 1020 |
| 13C chemical shifts | 680 |
| 15N chemical shifts | 157 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SopE2 monomer | 1 |
Entities:
Entity 1, SopE2 monomer 172 residues - 18398.1 Da.
| 1 | GLY | ASN | ALA | SER | GLU | GLY | ARG | ALA | VAL | LEU | ||||
| 2 | THR | SER | LYS | THR | VAL | LYS | ASP | PHE | MET | LEU | ||||
| 3 | GLN | LYS | LEU | ASN | SER | LEU | ASP | ILE | LYS | GLY | ||||
| 4 | ASN | ALA | SER | LYS | ASP | PRO | ALA | TYR | ALA | ARG | ||||
| 5 | GLN | THR | CYS | GLU | ALA | ILE | LEU | SER | ALA | VAL | ||||
| 6 | TYR | SER | ASN | ASN | LYS | ASP | GLN | CYS | CYS | LYS | ||||
| 7 | LEU | LEU | ILE | SER | LYS | GLY | VAL | SER | ILE | THR | ||||
| 8 | PRO | PHE | LEU | LYS | GLU | ILE | GLY | GLU | ALA | ALA | ||||
| 9 | GLN | ASN | ALA | GLY | LEU | PRO | GLY | GLU | ILE | LYS | ||||
| 10 | ASN | GLY | VAL | PHE | THR | PRO | GLY | GLY | ALA | GLY | ||||
| 11 | ALA | ASN | PRO | PHE | VAL | VAL | PRO | LEU | ILE | ALA | ||||
| 12 | SER | ALA | SER | ILE | LYS | TYR | PRO | HIS | MET | PHE | ||||
| 13 | ILE | ASN | HIS | ASN | GLN | GLN | VAL | SER | PHE | LYS | ||||
| 14 | ALA | TYR | ALA | GLU | LYS | ILE | VAL | MET | LYS | GLU | ||||
| 15 | VAL | THR | PRO | LEU | PHE | ASN | LYS | GLY | THR | MET | ||||
| 16 | PRO | THR | PRO | GLN | GLN | PHE | GLN | LEU | THR | ILE | ||||
| 17 | GLU | ASN | ILE | ALA | ASN | LYS | TYR | LEU | GLN | ASN | ||||
| 18 | ALA | SER |
Samples:
sample_1: Salmonella SopE2, [U-95% 13C; U-90% 15N], 0.5 – 1.0 mM
Ex-cond_1: pH: 7.0 na; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | not available |
| 1H-1H NOESY | not available | not available | not available |
| 1H-1H TOCSY | not available | not available | not available |
| 1H-15N TOCSY | not available | not available | not available |
| 1H-15N NOESY | not available | not available | not available |
| HNCA | not available | not available | not available |
| HNCACB | not available | not available | not available |
| HNHA | not available | not available | not available |
| HNHB | not available | not available | not available |
| CBCACONH | not available | not available | not available |
| HNCO | not available | not available | not available |
| HNCACO | not available | not available | not available |
| HNCOCA | not available | not available | not available |
| HCCONH | not available | not available | not available |
| CCONH | not available | not available | not available |
| HAHBCBCACONNH | not available | not available | not available |
| HCCH-COSY | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
| NH2-SELECTIVE 1H-15N HSQC | not available | not available | not available |
| CN NOESY | not available | not available | not available |
Software:
SPARKY - peak analysis
NMR spectrometers:
- VARIAN INOVA 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAJ36820 BAP07723 |
| EMBL | CAR32763 CAR37140 CAR59099 CBG24854 CBW17878 |
| GB | AAF63159 AAF91225 AAK27350 AAK27351 AAL20770 |
| REF | NP_460811 WP_000182065 WP_000182066 WP_000182067 WP_000182068 |
| SP | Q5PHN0 Q7CQD4 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts