BMRB Entry 5753
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5753
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Title: Towards an Understanding of the Poliovirus Replication Complex: The Solution Structure of the Soluble Domain of the Poliovirus 3A Protein PubMed: 12823963
Deposition date: 2003-03-21 Original release date: 2003-08-08
Authors: Strauss, D.; Glustrom, L.; Wuttke, D.
Citation: Strauss, D.; Glustrom, L.; Wuttke, D.. "Towards an Understanding of the Poliovirus Replication Complex: The Solution Structure of the Soluble Domain of the Poliovirus 3A Protein" J. Mol. Biol. 330, 225-234 (2003).
Assembly members:
Poliovirus 3A, polymer, 60 residues, Formula weight is not available
Natural source: Common Name: Poliovirus Taxonomy ID: 138953 Superkingdom: Viruses Kingdom: not available Genus/species: Enterovirus poliovirus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Poliovirus 3A: MGPLQYKDLKIDIKTSPPPE
CINDLLQAVDSQEVRDYCEK
KGWIVNITSQVQTERNINRA
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 390 |
| 13C chemical shifts | 253 |
| 15N chemical shifts | 67 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | 3A monomer, chain A | 1 |
| 2 | 3A monomer, chain B | 1 |
Entities:
Entity 1, 3A monomer, chain A 60 residues - Formula weight is not available
| 1 | MET | GLY | PRO | LEU | GLN | TYR | LYS | ASP | LEU | LYS | |
| 2 | ILE | ASP | ILE | LYS | THR | SER | PRO | PRO | PRO | GLU | |
| 3 | CYS | ILE | ASN | ASP | LEU | LEU | GLN | ALA | VAL | ASP | |
| 4 | SER | GLN | GLU | VAL | ARG | ASP | TYR | CYS | GLU | LYS | |
| 5 | LYS | GLY | TRP | ILE | VAL | ASN | ILE | THR | SER | GLN | |
| 6 | VAL | GLN | THR | GLU | ARG | ASN | ILE | ASN | ARG | ALA |
Samples:
sample_1: Poliovirus 3A, [U-95% 13C; U-99% 15N], 1.5 mM; potassium phosphate 20 mM; NaCl 50 mM; D2O 100%
sample_2: Poliovirus 3A, [U-95% 13C;U-99% 15N], 2.0 mM; potassium phosphate 20 mM; NaCl 50 mM; H2O 95%; D2O 5%
sample_cond_1: pH: 7.0; temperature: 298 K; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | not available | not available | not available |
| 2D NOESY | not available | not available | not available |
| 3D 15N-separated NOESY | not available | not available | not available |
| 3D 13C-separated select/filter NOESY | not available | not available | not available |
Software:
VNMR v6.1a - collection
NMRPipe v1.0 - processing
ANSIG v3.3 - data analysis
X-PLOR v3.851 - refinement
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| PDB | |
| EMBL | CAA24445 CAA24446 CAA24456 CAA24461 CAA24465 |
| GB | AAA46911 AAA46914 AAL75970 AAM09803 AAM09805 |
| PRF | 0707268A 0908283A |
| REF | NP_041277 NP_740474 YP_007353733 |
| SP | P03300 P03301 P03302 P06209 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts