BMRB Entry 5796
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5796
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Title: Solution Structure of a Putative Ribosome Binding-Protein from Mycoplasma pneumoniae and Comparison to a Distant Homolog PubMed: 15185964
Deposition date: 2003-05-13 Original release date: 2004-10-18
Authors: Rubin, Seth; Pelton, Jeffery; Yokota, Hisao; Kim, Rosalind; Wemmer, David
Citation: Rubin, Seth; Pelton, Jeffery; Yokota, Hisao; Kim, Rosalind; Wemmer, David. "Solution Structure of a Putative Ribosome Binding Protein from Mycoplasma pneumoniae and Comparison to a Distant Homolog " J. Struct. Funct. Genomics 4, 235-243 (2003).
Assembly members:
MPN156, polymer, 117 residues, Formula weight is not available
Natural source: Common Name: Mycoplasma pneumoniae Taxonomy ID: 2104 Superkingdom: Eubacteria Kingdom: not available Genus/species: Mycoplasma pneumoniae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
MPN156: GMASYKKERLENDIIRLINR
TVIHEIYNETVKTGHVTHVK
LSDDLLHVTVYLDCYNREQI
DRVVGAFNQAKGVFSRVLAH
NLYLAKAVQIHFVKDKAIDN
AMRIESIINSLKKSKPN
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 1H chemical shifts | 586 |
| 13C chemical shifts | 307 |
| 15N chemical shifts | 96 |
| coupling constants | 65 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | MPN156 | 1 |
Entities:
Entity 1, MPN156 117 residues - Formula weight is not available
| 1 | GLY | MET | ALA | SER | TYR | LYS | LYS | GLU | ARG | LEU | ||||
| 2 | GLU | ASN | ASP | ILE | ILE | ARG | LEU | ILE | ASN | ARG | ||||
| 3 | THR | VAL | ILE | HIS | GLU | ILE | TYR | ASN | GLU | THR | ||||
| 4 | VAL | LYS | THR | GLY | HIS | VAL | THR | HIS | VAL | LYS | ||||
| 5 | LEU | SER | ASP | ASP | LEU | LEU | HIS | VAL | THR | VAL | ||||
| 6 | TYR | LEU | ASP | CYS | TYR | ASN | ARG | GLU | GLN | ILE | ||||
| 7 | ASP | ARG | VAL | VAL | GLY | ALA | PHE | ASN | GLN | ALA | ||||
| 8 | LYS | GLY | VAL | PHE | SER | ARG | VAL | LEU | ALA | HIS | ||||
| 9 | ASN | LEU | TYR | LEU | ALA | LYS | ALA | VAL | GLN | ILE | ||||
| 10 | HIS | PHE | VAL | LYS | ASP | LYS | ALA | ILE | ASP | ASN | ||||
| 11 | ALA | MET | ARG | ILE | GLU | SER | ILE | ILE | ASN | SER | ||||
| 12 | LEU | LYS | LYS | SER | LYS | PRO | ASN |
Samples:
sample_15N: MPN156, [U-100% 15N], 1 mM; sodium phosphate 25 mM; sodium chloride 50 mM; DTT 1 mM; EDTA 1 mM
sample_15N_13C: MPN156, [U-100% 15N; U-100% 13C], 1 mM; sodium phosphate 25 mM; sodium chloride 50 mM; DTT 1 mM; EDTA 1 mM
sample_13C: MPN156, [U-10% 13C], 1 mM; sodium phosphate 25 mM; sodium chloride 50 mM; DTT 1 mM; EDTA 1 mM
Sample_conditions: pH: 6.6 na; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HSQC | not available | not available | not available |
| HNCA | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| HBHA(CO)NH | not available | not available | not available |
| HNHA | not available | not available | not available |
| 15-N NOESY | not available | not available | not available |
| HCCH TOCSY | not available | not available | not available |
Software:
NMRPIPE v1.0 - Processing
NMRVIEW v5.0.4 - Analysis
NMR spectrometers:
- BRUKER DRX 500 MHz
- BRUKER DRX 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAL21730 |
| GB | AAB96323 ADK86785 AGC04083 |
| REF | NP_109844 WP_010874513 YP_005175394 YP_005881189 YP_007347770 |
| SP | P75589 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts