BMRB Entry 5896
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5896
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Title: Unusual Ca2+-calmodulin binding interactions of the microtubule-associated protein F-STOP PubMed: 14516200
Deposition date: 2003-08-05 Original release date: 2004-02-11
Authors: Bouvier, Denis; Vanhaverbeke, Cecile; Simorre, Jean-Pierre; Gans, Pierre
Citation: Bouvier, Denis; Vanhaverbeke, Cecile; Simorre, Jean-Pierre; Arlaud, Gerard; Bally, Isabelle; Forge, Vincent; Margolis, Robert; Gans, Pierre; Kleman, Jean-Philippe. "Unusual Ca(2+)-calmodulin Binding Interactions of the Microtubule-associated Protein F-STOP" Biochemistry 42, 11484-11493 (2003).
Assembly members:
calmodulin, polymer, 163 residues, 18522 Da.
F-STOP Mc module, polymer, 23 residues, 2739 Da.
CALCIUM ION, non-polymer, 40.078 Da.
Natural source: Common Name: rabbit Taxonomy ID: 9986 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Oryctolagus cuniculus
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
calmodulin: MSHHHHHHSMDIEFMADQLT
DEQIAEFKEAFSLFDKDGDG
TITTKELGTVMRSLGQNPTE
AELQDMINEVDADGNGTIDF
PEFLNLMARKMKDTDSEEEL
KEAFRVFDKDGNGFISAAEL
RHVMTNLGEKLTDEEVDEMI
READVDGDGQVNYEEFVQVM
MAK
F-STOP Mc module: QRDTRRKAGPAWMVTRTEGH
EEK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 152 |
| 13C chemical shifts | 153 |
| 15N chemical shifts | 152 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | calmodulin | 1 |
| 2 | F-STOP Mc module | 2 |
| 3 | CALCIUM (II) ION | 3 |
Entities:
Entity 1, calmodulin 163 residues - 18522 Da.
| 1 | MET | SER | HIS | HIS | HIS | HIS | HIS | HIS | SER | MET | ||||
| 2 | ASP | ILE | GLU | PHE | MET | ALA | ASP | GLN | LEU | THR | ||||
| 3 | ASP | GLU | GLN | ILE | ALA | GLU | PHE | LYS | GLU | ALA | ||||
| 4 | PHE | SER | LEU | PHE | ASP | LYS | ASP | GLY | ASP | GLY | ||||
| 5 | THR | ILE | THR | THR | LYS | GLU | LEU | GLY | THR | VAL | ||||
| 6 | MET | ARG | SER | LEU | GLY | GLN | ASN | PRO | THR | GLU | ||||
| 7 | ALA | GLU | LEU | GLN | ASP | MET | ILE | ASN | GLU | VAL | ||||
| 8 | ASP | ALA | ASP | GLY | ASN | GLY | THR | ILE | ASP | PHE | ||||
| 9 | PRO | GLU | PHE | LEU | ASN | LEU | MET | ALA | ARG | LYS | ||||
| 10 | MET | LYS | ASP | THR | ASP | SER | GLU | GLU | GLU | LEU | ||||
| 11 | LYS | GLU | ALA | PHE | ARG | VAL | PHE | ASP | LYS | ASP | ||||
| 12 | GLY | ASN | GLY | PHE | ILE | SER | ALA | ALA | GLU | LEU | ||||
| 13 | ARG | HIS | VAL | MET | THR | ASN | LEU | GLY | GLU | LYS | ||||
| 14 | LEU | THR | ASP | GLU | GLU | VAL | ASP | GLU | MET | ILE | ||||
| 15 | ARG | GLU | ALA | ASP | VAL | ASP | GLY | ASP | GLY | GLN | ||||
| 16 | VAL | ASN | TYR | GLU | GLU | PHE | VAL | GLN | VAL | MET | ||||
| 17 | MET | ALA | LYS |
Entity 2, F-STOP Mc module 23 residues - 2739 Da.
| 1 | GLN | ARG | ASP | THR | ARG | ARG | LYS | ALA | GLY | PRO | ||||
| 2 | ALA | TRP | MET | VAL | THR | ARG | THR | GLU | GLY | HIS | ||||
| 3 | GLU | GLU | LYS |
Entity 3, CALCIUM (II) ION - Ca - 40.078 Da.
| 1 | CA |
Samples:
sample_1: calmodulin, [U-97% 13C; U-98% 15N], 0.7 mM; F-STOP Mc module 0.7 mM
Condition_1: pH: 7.7; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | sample_1 | not available | Condition_1 |
| HNCA | sample_1 | not available | Condition_1 |
Software:
FELIX v2000 - raw spectral data processing, peak picking
NMR spectrometers:
- Varian Inova 600 MHz
- Varian Inova 800 MHz
Related Database Links:
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts