BMRB Entry 6023
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6023
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Title: 1H, 15N, and 13C Resonance Assignments of Calmodulin Complexed with the Calmodulin-Binding Domain of Protein Phosphatase 2B PubMed: 15243184
Deposition date: 2003-11-28 Original release date: 2004-12-01
Authors: Lin, Ta-Hsien; Huang, Jian-Wen; Huang, Hsien-bin; Chen, Yi-chen; Liu, Chia-Yen; Lo, Chi-jen; Tang, Tzu-Chun; Chyan, Chia-Lin
Citation: Lin, Ta-Hsien; Huang, Jian-Wen; Huang, Hsien-bin; Chen, Yi-chen; Liu, Chia-Yen; Lo, Chi-jen; Tang, Tzu; Chyan, Chia-Lin. "Letter to the Editor: 1H, 15N, and 13C Resonance Assignments of Calmodulin Complexed with the Calmodulin-Binding Domain of calcineurin" J. Biomol. NMR 29, 531-532 (2004).
Assembly members:
Calmodulin, polymer, 148 residues, 16706 Da.
protein phosphatase 2B peptide, polymer, 24 residues, 2812 Da.
Natural source: Common Name: Chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Calmodulin: ADQLTEEQIAEFKEAFSLFD
KDGDGTITTKELGTVMRSLG
QNPTEAELQDMINEVDADGN
GTIDFPEFLTMMARKMKDTD
SEEEIREAFRVFDKDGNGYI
SAAELRHVMTNLGEKLTDEE
VDEMIREADIDGDGQVNYEE
FVQMMTAK
protein phosphatase 2B peptide: ARKEVIRNKIRAIGKMARVF
SVLR
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 837 |
| 13C chemical shifts | 565 |
| 15N chemical shifts | 142 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Calmodulin | 1 |
| 2 | Protein Phosphatase 2B | 2 |
Entities:
Entity 1, Calmodulin 148 residues - 16706 Da.
| 1 | ALA | ASP | GLN | LEU | THR | GLU | GLU | GLN | ILE | ALA | ||||
| 2 | GLU | PHE | LYS | GLU | ALA | PHE | SER | LEU | PHE | ASP | ||||
| 3 | LYS | ASP | GLY | ASP | GLY | THR | ILE | THR | THR | LYS | ||||
| 4 | GLU | LEU | GLY | THR | VAL | MET | ARG | SER | LEU | GLY | ||||
| 5 | GLN | ASN | PRO | THR | GLU | ALA | GLU | LEU | GLN | ASP | ||||
| 6 | MET | ILE | ASN | GLU | VAL | ASP | ALA | ASP | GLY | ASN | ||||
| 7 | GLY | THR | ILE | ASP | PHE | PRO | GLU | PHE | LEU | THR | ||||
| 8 | MET | MET | ALA | ARG | LYS | MET | LYS | ASP | THR | ASP | ||||
| 9 | SER | GLU | GLU | GLU | ILE | ARG | GLU | ALA | PHE | ARG | ||||
| 10 | VAL | PHE | ASP | LYS | ASP | GLY | ASN | GLY | TYR | ILE | ||||
| 11 | SER | ALA | ALA | GLU | LEU | ARG | HIS | VAL | MET | THR | ||||
| 12 | ASN | LEU | GLY | GLU | LYS | LEU | THR | ASP | GLU | GLU | ||||
| 13 | VAL | ASP | GLU | MET | ILE | ARG | GLU | ALA | ASP | ILE | ||||
| 14 | ASP | GLY | ASP | GLY | GLN | VAL | ASN | TYR | GLU | GLU | ||||
| 15 | PHE | VAL | GLN | MET | MET | THR | ALA | LYS |
Entity 2, Protein Phosphatase 2B 24 residues - 2812 Da.
| 1 | ALA | ARG | LYS | GLU | VAL | ILE | ARG | ASN | LYS | ILE | ||||
| 2 | ARG | ALA | ILE | GLY | LYS | MET | ALA | ARG | VAL | PHE | ||||
| 3 | SER | VAL | LEU | ARG |
Samples:
sample_1: Calmodulin, [U-99% 13C; U-99% 15N], 1.1 mM; protein phosphatase 2B peptide 1.1 mM; KCl 100 mM; CaCl2 5 mM; D2O 10%; H2O 90%
Ex-cond_1: pH: 6.5; temperature: 310 K; ionic strength: 0.13 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCA | not available | not available | not available |
| HN(CO)CA | not available | not available | not available |
| HNCO | not available | not available | not available |
| HN(CA)CO | not available | not available | not available |
| CBCANH | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| 15N-HSQC | not available | not available | not available |
| HBHA(CACBCO)NH | not available | not available | not available |
| H(CCO)NH | not available | not available | not available |
| C(CO)NH | not available | not available | not available |
| 1H-15N HSQC-TOCSY | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker AVANCE 500 MHz
- Bruker AVANCE 600 MHz
Related Database Links:
| BMRB | 15624 |
| PDB | |
| DBJ | BAA14083 BAB19673 BAC36398 BAE27131 BAE29521 BAE29521 BAE27131 BAC36398 BAB19673 BAA14083 |
| EMBL | CAA40398 CAA54807 CAB89253 CAF94324 CAH91746 CAH91746 CAF94324 CAB89253 CAA54807 CAA40398 |
| GB | AAA02631 AAA35634 AAA37359 AAA37432 AAA40940 |
| PRF | 1714202A 2006264A 2006264A 1714202A |
| REF | NP_000935 NP_001074063 NP_001076161 NP_001080813 NP_001087372 NP_001087372 NP_001080813 NP_001076161 NP_001074063 NP_000935 |
| SP | P48452 P63328 P63329 Q08209 Q27889 |
| TPG | DAA28842 |
| SWISS-PROT | Q27889 Q08209 P63329 P63328 P48452 |
| GenBank | AAA40940 AAA37432 AAA37359 AAA35634 AAA02631 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts