BMRB Entry 6064
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6064
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Title: E6-binding zinc finger (E6apc1) PubMed: 15182185
Deposition date: 2004-01-08 Original release date: 2008-07-17
Authors: Liu, Y.; Liu, Z.; Androphy, E.; Chen, J.; Baleja, J.
Citation: Liu, Y.; Liu, Z.; Androphy, E.; Chen, J.; Baleja, J.. "DESIGN AND CHARACTERIZATION OF HELICAL PEPTIDES THAT INHIBIT THE E6 PROTEIN OF PAPILLOMAVIRUS" Biochemistry 43, 7421-7431 (2004).
Assembly members:
E6apc1, polymer, 31 residues, Formula weight is not available
Natural source: Common Name: Papillomavirus Taxonomy ID: 10558 Superkingdom: Viruses Kingdom: not available Genus/species: Papillomavirus not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
E6apc1: XYKFACPECPKRFMRSDHLTL
HILLHENKKX
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 166 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | E6apc1 peptide | 1 |
Entities:
Entity 1, E6apc1 peptide 31 residues - Formula weight is not available
| 1 | ACE | TYR | LYS | PHE | ALA | CYS | PRO | GLU | CYS | PRO | ||||
| 2 | LYS | ARG | PHE | MET | ARG | SER | ASP | HIS | LEU | THR | ||||
| 3 | LEU | HIS | ILE | LEU | LEU | HIS | GLU | ASN | LYS | LYS | ||||
| 4 | NH2 |
Samples:
sample_1: E6apc11.2 – 3.0 mM; imidazole, [U-2H], 10 mM; ZnSO4 4 mM; DTT 1 mM; H2O 90%; D2O 10%
sample_2: E6apc11.2 – 3.0 mM; imidazole, [U-2H], 10 mM; ZnSO4 4 mM; DTT 1 mM; D2O 99.96%
sample_cond_1: ionic strength: 4 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D TOCSY | not available | not available | sample_cond_1 |
| 2D NOESY | not available | not available | sample_cond_1 |
| DQF-COSY | not available | not available | sample_cond_1 |
Software:
xwinnmr v3.5 - collection, processing
CNS v1.1 - refinement, structure solution
NMR spectrometers:
- Bruker AMX 500 MHz
Related Database Links:
| PDB |