BMRB Entry 6087
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6087
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Title: Conformation and Ion-Channeling activity of the hepatitis C virus p7 polypeptide PubMed: 20667830
Deposition date: 2004-01-29 Original release date: 2005-01-20
Authors: Montserret, Roland; Saint, Nathalie; Simorre, Jean Pierre; Penin, Francois
Citation: Montserret, Roland; Saint, Nathalie; Vanbelle, Christophe; Salvay, Andres; Simorre, Jean-Pierre; Ebel, Christine; Sapay, Nicolas; Renisio, Jean-Guillaume; Bockmann, Anja; Steinmann, Eike; Pietschmann, Thomas; Dubuisson, Jean; Chipot, Christophe; Penin, Francois. "NMR structure and ion channel activity of the p7 protein from hepatitis C virus" J. Biol. Chem. ., .-. (2010).
Assembly members:
p7, polymer, 63 residues, 6698.16 Da.
Natural source: Common Name: HCV Taxonomy ID: 11103 Superkingdom: Viruses Kingdom: not available Genus/species: Hepacivirus Hepatitis C virus
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
p7: TLENLVVLNAASVAGAHGLL
SFLVFFAAAWYIKGRLVPGA
AYALYGVWPLLLLLLALPPR
AYA
- assigned_chemical_shifts
Data type | Count |
1H chemical shifts | 472 |
13C chemical shifts | 167 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | HCV p7 | 1 |
Entities:
Entity 1, HCV p7 63 residues - 6698.16 Da.
1 | THR | LEU | GLU | ASN | LEU | VAL | VAL | LEU | ASN | ALA | ||||
2 | ALA | SER | VAL | ALA | GLY | ALA | HIS | GLY | LEU | LEU | ||||
3 | SER | PHE | LEU | VAL | PHE | PHE | ALA | ALA | ALA | TRP | ||||
4 | TYR | ILE | LYS | GLY | ARG | LEU | VAL | PRO | GLY | ALA | ||||
5 | ALA | TYR | ALA | LEU | TYR | GLY | VAL | TRP | PRO | LEU | ||||
6 | LEU | LEU | LEU | LEU | LEU | ALA | LEU | PRO | PRO | ARG | ||||
7 | ALA | TYR | ALA |
Samples:
sample_1: p7 1.26 mM; 2,2,2-trifluoroethanol-d2 90%; H2O 10%
condition_1: pH: 3.0; temperature: 301 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
NOESY | not available | not available | not available |
TOCSY | not available | not available | not available |
1H-13C-HSQC | not available | not available | not available |
HSQC-TOCSY in 13C natural abondancy. | not available | not available | not available |
Software:
vnmr v6.1 - acquisition, processing
Sparky v3.106 - Analysis
NMR spectrometers:
- Varian Unity-plus 500 MHz
- Varian Inova 800 MHz