BMRB Entry 6100
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6100
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Title: Backbone 1H, 13C and 15N chemical shift assignents of the 18.5kDa isoform of murine myelin basic protein (MBP) PubMed: 15243191
Deposition date: 2004-02-12 Original release date: 2007-10-19
Authors: Libich, David; Robertson, Valerie; Monette, Martine; Harauz, George
Citation: Libich, David; Robertson, Valerie; Monette, Martine; Harauz, George. "Letter to the Editor: Backbone resonance assignments of the 18.5kDa isoform of murine myelin basic protein (MBP)" J. Biomol. NMR 29, 545-546 (2004).
Assembly members:
myelin basic protein, polymer, 176 residues, 19421.5 Da.
Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
myelin basic protein: ASQKRPSQRSKYLATASTMD
HARHGFLPRHRDTGILDSIG
RFFSGDRGAPKRGSGKDSHT
RTTHYGSLPQKSQHGRTQDE
NPVVHFFKNIVTPRTPPPSQ
GKGRGLSLSRFSWGAEGQRP
GFGYGGRASDYKSAHKGFKG
AYDAQGTLSKIFKLGGRDSR
SGSPMARRLEHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 436 |
| 15N chemical shifts | 154 |
| 1H chemical shifts | 516 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | rmMBP | 1 |
Entities:
Entity 1, rmMBP 176 residues - 19421.5 Da.
| 1 | ALA | SER | GLN | LYS | ARG | PRO | SER | GLN | ARG | SER | ||||
| 2 | LYS | TYR | LEU | ALA | THR | ALA | SER | THR | MET | ASP | ||||
| 3 | HIS | ALA | ARG | HIS | GLY | PHE | LEU | PRO | ARG | HIS | ||||
| 4 | ARG | ASP | THR | GLY | ILE | LEU | ASP | SER | ILE | GLY | ||||
| 5 | ARG | PHE | PHE | SER | GLY | ASP | ARG | GLY | ALA | PRO | ||||
| 6 | LYS | ARG | GLY | SER | GLY | LYS | ASP | SER | HIS | THR | ||||
| 7 | ARG | THR | THR | HIS | TYR | GLY | SER | LEU | PRO | GLN | ||||
| 8 | LYS | SER | GLN | HIS | GLY | ARG | THR | GLN | ASP | GLU | ||||
| 9 | ASN | PRO | VAL | VAL | HIS | PHE | PHE | LYS | ASN | ILE | ||||
| 10 | VAL | THR | PRO | ARG | THR | PRO | PRO | PRO | SER | GLN | ||||
| 11 | GLY | LYS | GLY | ARG | GLY | LEU | SER | LEU | SER | ARG | ||||
| 12 | PHE | SER | TRP | GLY | ALA | GLU | GLY | GLN | ARG | PRO | ||||
| 13 | GLY | PHE | GLY | TYR | GLY | GLY | ARG | ALA | SER | ASP | ||||
| 14 | TYR | LYS | SER | ALA | HIS | LYS | GLY | PHE | LYS | GLY | ||||
| 15 | ALA | TYR | ASP | ALA | GLN | GLY | THR | LEU | SER | LYS | ||||
| 16 | ILE | PHE | LYS | LEU | GLY | GLY | ARG | ASP | SER | ARG | ||||
| 17 | SER | GLY | SER | PRO | MET | ALA | ARG | ARG | LEU | GLU | ||||
| 18 | HIS | HIS | HIS | HIS | HIS | HIS |
Samples:
sample_1: myelin basic protein, [U-15N; U-13C], 2 mM; 2,2,2-trifluoroethanol-d2, [2H], 30%
sample_cond_1: pH: 6.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N-HSQC | sample_1 | not available | sample_cond_1 |
| CBCANH | sample_1 | not available | sample_cond_1 |
| CBCA(CO)NH | sample_1 | not available | sample_cond_1 |
| HCC(CO)NH | sample_1 | not available | sample_cond_1 |
| HNCO | sample_1 | not available | sample_cond_1 |
Software:
No software information available
NMR spectrometers:
- Bruker AV 600 MHz
Related Database Links:
| BMRB | 15131 |
| GB | AAA37720 AAA39496 AAA39501 EDL09385 |
| REF | NP_001020426 NP_001020463 NP_034907 XP_006526519 |
| SP | P04370 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts