BMRB Entry 6221
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6221
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments of the Resuscitation Promoting Factor (Rpf) domain of M. tuberculosis PubMed: 15614636
Deposition date: 2004-05-27 Original release date: 2004-12-16
Authors: Cohen-Gonsaud, Martin; Barthe, Philippe; Keep, Nicholas; Roumestand, Christian
Citation: Cohen-Gonsaud, Martin; Barthe, Philippe; Pommier, Francoise; Harris, Richard; Driscoll, Paul; Keep, Nicholas; Roumestand, Christian. "1H, 15N and 13C Chemical Shifts Assignments of the Resuscitation Promoting Factor domain of Rv1009 from Mycobacterium tuberculosis" J. Biomol. NMR 30, 373-374 (2004).
Assembly members:
Rpf domain, polymer, 108 residues, Formula weight is not available
Natural source: Common Name: Mycobacterium tuberculosis Taxonomy ID: 83332 Superkingdom: Actinobacteria Kingdom: Actinomycetales Genus/species: Mycobacterium tuberculosis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Rpf domain: NVVVTPAHEAVVRVGTKPGT
EVPPVIDGSIWDAIAGCEAG
GNWAINTGNGYYGGVQFDQG
TWEANGGLRYAPRADLATRE
EQIAVAEVTRLRQGWGAWPV
CAARAGAR
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 300 |
| 15N chemical shifts | 124 |
| 1H chemical shifts | 677 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RpfBc domain from M. tuberculosis | 1 |
Entities:
Entity 1, RpfBc domain from M. tuberculosis 108 residues - Formula weight is not available
| 1 | ASN | VAL | VAL | VAL | THR | PRO | ALA | HIS | GLU | ALA | ||||
| 2 | VAL | VAL | ARG | VAL | GLY | THR | LYS | PRO | GLY | THR | ||||
| 3 | GLU | VAL | PRO | PRO | VAL | ILE | ASP | GLY | SER | ILE | ||||
| 4 | TRP | ASP | ALA | ILE | ALA | GLY | CYS | GLU | ALA | GLY | ||||
| 5 | GLY | ASN | TRP | ALA | ILE | ASN | THR | GLY | ASN | GLY | ||||
| 6 | TYR | TYR | GLY | GLY | VAL | GLN | PHE | ASP | GLN | GLY | ||||
| 7 | THR | TRP | GLU | ALA | ASN | GLY | GLY | LEU | ARG | TYR | ||||
| 8 | ALA | PRO | ARG | ALA | ASP | LEU | ALA | THR | ARG | GLU | ||||
| 9 | GLU | GLN | ILE | ALA | VAL | ALA | GLU | VAL | THR | ARG | ||||
| 10 | LEU | ARG | GLN | GLY | TRP | GLY | ALA | TRP | PRO | VAL | ||||
| 11 | CYS | ALA | ALA | ARG | ALA | GLY | ALA | ARG |
Samples:
sample_1: Rpf domain, [U-95% 15N], 0.5 mM; Na-Acetate 25 mM; beta-mercaptoethanol 5 mM
sample_2: Rpf domain, [U-95% 13C; U-95% 15N], 0.5 mM; Na-Acetate 25 mM; beta-mercaptoethanol 5 mM
Ex-cond_1: ionic strength: 25 mM; pH: 4.6; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCA | not available | not available | Ex-cond_1 |
| HN(CO)CA | not available | not available | Ex-cond_1 |
| HNCACB | not available | not available | Ex-cond_1 |
| CBCA(CO)NH | not available | not available | Ex-cond_1 |
| HNCO | not available | not available | Ex-cond_1 |
| HN(CA)CO | not available | not available | Ex-cond_1 |
| 1H-15N NOESY | not available | not available | Ex-cond_1 |
| 1H-15N TOCSY | not available | not available | Ex-cond_1 |
Software:
No software information available
NMR spectrometers:
- Bruker Avance 500 MHz
Related Database Links:
| PDB | |
| DBJ | BAH25329 |
| EMBL | CAB08136 CAD93897 CAL71053 |
| GB | AAK45288 ABQ72752 ABR05371 ACT26067 EAY59373 |
| REF | NP_215525 NP_335474 NP_854693 YP_001282314 YP_001286973 |
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