BMRB Entry 6236
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6236
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for CAPPD*, an Independently Folded Extracellular Domain of Human Amyloid-beta Precursor Protein PubMed: 15274612
Deposition date: 2004-06-11 Original release date: 2004-09-01
Authors: Dulubova, Irina; Ho, Angela; Huryeva, Iryna; Sudhof, Thomas; Rizo, Josep
Citation: Dulubova, Irina; Ho, Angela; Huryeva, Iryna; Sudhof, Thomas; Rizo, Josep. "Three-Dimensional Structure of an Independently Folded Extracellular Domain of Human Amyloid-beta Precursor Protein" Biochemistry 43, 9583-9588 (2004).
Assembly members:
Amyloid-beta Precursor Protein, polymer, 117 residues, Formula weight is not available
Natural source: Common Name: 9606 Taxonomy ID: Human Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo Sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Amyloid-beta Precursor Protein: RVEAMLNDRRRLALENYITA
LQAVPPRPRHVFNMLKKYVR
AEQKDRQHTLKHFEHVRMVD
PKKAAQIRSQVMTHLRVIYE
RMNQSLSLLYNVPAVAEEIQ
DEVDELLQKEQNYSDDV
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 897 |
| 13C chemical shifts | 545 |
| 15N chemical shifts | 129 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | APP fragment, CAPPD* | 1 |
Entities:
Entity 1, APP fragment, CAPPD* 117 residues - Formula weight is not available
| 1 | ARG | VAL | GLU | ALA | MET | LEU | ASN | ASP | ARG | ARG | ||||
| 2 | ARG | LEU | ALA | LEU | GLU | ASN | TYR | ILE | THR | ALA | ||||
| 3 | LEU | GLN | ALA | VAL | PRO | PRO | ARG | PRO | ARG | HIS | ||||
| 4 | VAL | PHE | ASN | MET | LEU | LYS | LYS | TYR | VAL | ARG | ||||
| 5 | ALA | GLU | GLN | LYS | ASP | ARG | GLN | HIS | THR | LEU | ||||
| 6 | LYS | HIS | PHE | GLU | HIS | VAL | ARG | MET | VAL | ASP | ||||
| 7 | PRO | LYS | LYS | ALA | ALA | GLN | ILE | ARG | SER | GLN | ||||
| 8 | VAL | MET | THR | HIS | LEU | ARG | VAL | ILE | TYR | GLU | ||||
| 9 | ARG | MET | ASN | GLN | SER | LEU | SER | LEU | LEU | TYR | ||||
| 10 | ASN | VAL | PRO | ALA | VAL | ALA | GLU | GLU | ILE | GLN | ||||
| 11 | ASP | GLU | VAL | ASP | GLU | LEU | LEU | GLN | LYS | GLU | ||||
| 12 | GLN | ASN | TYR | SER | ASP | ASP | VAL |
Samples:
sample_1: Amyloid-beta Precursor Protein, [U-99% 15N], 0.85 mM; sodium phosphate 50 mM; NaCl 250 mM; Guanidinium Chloride 300 mM; D2O 5%
sample_2: Amyloid-beta Precursor Protein, [U-99% 15N; U-13C], 0.85 mM; sodium phosphate 50 mM; NaCl 250 mM; Guanidinium Chloride 300 mM; D2O 5%
Ex-cond_1: pH: 6.4; temperature: 300 K; ionic strength: 0.6 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-15N NOESY-HSQC and TOCSY-HSQC | not available | not available | not available |
| HNCO, HNCACB | not available | not available | not available |
| H(C)(CO)NH-TOCSY | not available | not available | not available |
| (H)C(CO)NH-TOCSY | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
Software:
VNMR v6.1 -
NMR spectrometers:
- Varian INOVA 500 MHz
Related Database Links:
| PDB | |
| DBJ | BAA22264 BAA84580 BAC36369 BAD51938 BAE01907 |
| EMBL | CAA30050 CAA30488 CAA31830 CAA53090 CAA66230 |
| GB | AAA36829 AAA51722 AAA51726 AAA58727 AAB41502 |
| PRF | 1303338A 1403400A 1507304A 1507304B |
| REF | NP_000475 NP_001006601 NP_001013036 NP_001070264 NP_001127014 |
| SP | P05067 P08592 P12023 P53601 P79307 |
| TPG | DAA33655 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts