BMRB Entry 6315
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6315
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Title: 1H, 13C and 15N chemical shift assignments for TcUBP1 (GQ) (backbone + side chains) PubMed: 15751947
Deposition date: 2004-09-09 Original release date: 2005-05-03
Authors: Volpon, Laurent; D'orso, Ivan; Gehring, Kalle
Citation: Volpon, Laurent; D'orso, Ivan; Young, Christopher; Frasch, Alberto; Gehring, Kalle. "NMR structural study of TcUBP1, a single RRM domain protein from Trypanosoma cruzi: contribution of a beta hairpin to RNA binding" Biochemistry 44, 3708-3717 (2005).
Assembly members:
TcUBP1, polymer, 139 residues, 15413.4 Da.
Natural source: Common Name: Trypanosoma cruzi Taxonomy ID: 5693 Superkingdom: Eukaryota Kingdom: not available Genus/species: Trypanosoma cruzi
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
TcUBP1: MSQIPLVSQYDPYGQTAQLQ
QLQQQQQQHIPPTQMNPEPD
VLRNLMVNYIPTTVDEVQLR
QLFERYGPIESVKIVCDRET
RQSRGYGFVKFQSGSSAQQA
IAGLNGFNILNKRLKVALAA
SGHQRPGIAGAVGDGNGYL
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 757 |
| 13C chemical shifts | 486 |
| 15N chemical shifts | 123 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TcUBP1 | 1 |
Entities:
Entity 1, TcUBP1 139 residues - 15413.4 Da.
| 1 | MET | SER | GLN | ILE | PRO | LEU | VAL | SER | GLN | TYR | ||||
| 2 | ASP | PRO | TYR | GLY | GLN | THR | ALA | GLN | LEU | GLN | ||||
| 3 | GLN | LEU | GLN | GLN | GLN | GLN | GLN | GLN | HIS | ILE | ||||
| 4 | PRO | PRO | THR | GLN | MET | ASN | PRO | GLU | PRO | ASP | ||||
| 5 | VAL | LEU | ARG | ASN | LEU | MET | VAL | ASN | TYR | ILE | ||||
| 6 | PRO | THR | THR | VAL | ASP | GLU | VAL | GLN | LEU | ARG | ||||
| 7 | GLN | LEU | PHE | GLU | ARG | TYR | GLY | PRO | ILE | GLU | ||||
| 8 | SER | VAL | LYS | ILE | VAL | CYS | ASP | ARG | GLU | THR | ||||
| 9 | ARG | GLN | SER | ARG | GLY | TYR | GLY | PHE | VAL | LYS | ||||
| 10 | PHE | GLN | SER | GLY | SER | SER | ALA | GLN | GLN | ALA | ||||
| 11 | ILE | ALA | GLY | LEU | ASN | GLY | PHE | ASN | ILE | LEU | ||||
| 12 | ASN | LYS | ARG | LEU | LYS | VAL | ALA | LEU | ALA | ALA | ||||
| 13 | SER | GLY | HIS | GLN | ARG | PRO | GLY | ILE | ALA | GLY | ||||
| 14 | ALA | VAL | GLY | ASP | GLY | ASN | GLY | TYR | LEU |
Samples:
sample_1: TcUBP1, [U-13C; U-15N], 1.5 mM; Tris 50 mM; NaCl 50 mM; DTT 1 mM; NaN3 1 mM
sample_conditions: pH: 7.2; temperature: 298 K; pressure: 1 atm; ionic strength: 0.05 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| not available | sample_1 | not available | sample_conditions |
Software:
No software information available
NMR spectrometers:
- Bruker DRX 500 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts