BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6317

Title: 1H, 15N and 13C resonance assigment of the transcription factor CylR2 from Enterococcus faecalis   PubMed: 15359276

Deposition date: 2004-09-20 Original release date: 2004-12-22

Authors: Rumpel, Sigrun; Vijayan, Vinesh; Zweckstetter, Markus

Citation: Rumpel, Sigrun; Razeto, Adelia; Pillar, Chris; Vijayan, Vinesh; Taylor, Austin; Giller, Karin; Gilmore, Michael; Becker, Stefan; Zweckstetter, Markus. "Structure and DNA-binding properties of the cytolysin regulator CylR2 from Enterococcus faecalis"  EMBO J. 23, 3632-3642 (2004).

Assembly members:
cytolysin regulator R2, polymer, 66 residues, 15431 Da.

Natural source:   Common Name: Enterococcus faecalis   Taxonomy ID: 1351   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Enterococcus faecalis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
cytolysin regulator R2: MIINNLKLIREKKKISQSEL AALLEVSRQTINGIEKNKYN PSLQLALKIAYYLNTPLEDI FQWQPE

Data sets:
Data typeCount
13C chemical shifts327
15N chemical shifts73
1H chemical shifts522

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CylR2 subunit 11
2CylR2 subunit 21

Entities:

Entity 1, CylR2 subunit 1 66 residues - 15431 Da.

1   METILEILEASNASNLEULYSLEUILEARG
2   GLULYSLYSLYSILESERGLNSERGLULEU
3   ALAALALEULEUGLUVALSERARGGLNTHR
4   ILEASNGLYILEGLULYSASNLYSTYRASN
5   PROSERLEUGLNLEUALALEULYSILEALA
6   TYRTYRLEUASNTHRPROLEUGLUASPILE
7   PHEGLNTRPGLNPROGLU

Samples:

sample_1: cytolysin regulator R2, [U-95% 13C; U-90% 15N], 1.0 – 3.0 mM

cond_set_1: pH: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_1not availablecond_set_1

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

BMRB 17892 17893 17894 17895 17896 17897 17898
PDB
GB AAL59476 AAL60140 AAM75247 AAO80372 AIL03173
REF NP_814301 WP_002358483 WP_002370931 WP_010773902 YP_009077231

Download simulated HSQC data in one of the following formats:
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