BMRB Entry 6376
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6376
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Title: Complete resonance assignment of the C-terminal domain from TgMIC1
Deposition date: 2004-11-04 Original release date: 2005-03-18
Authors: Saouros, Savvas; Chen, Ho An; Cota, Ernesto; Edwards-Jones, Bryn; Simpson, Peter; Soldati-Favre, Dominique; Matthews, Stephen
Citation: Saouros, Savvas; Chen, Ho An; Simpson, Peter; Cota, Ernesto; Edwards-Jones, Bryn; Soldati-Favre, Dominique; Matthews, Stephen. "Letter to the Editor: Complete resonance assignments of the C-terminal domain from MIC1: A micronemal protein from Toxoplasma gondii" J. Biomol. NMR 31, 177-178 (2005).
Assembly members:
TgMIC1-CT, polymer, 137 residues, 14020 Da.
Natural source: Common Name: Toxoplasma gondii Taxonomy ID: 5811 Superkingdom: Eukaryota Kingdom: not available Genus/species: Toxoplasma gondii
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
TgMIC1-CT: KTEIHGDSTKATLEEGQQLT
LTFISTKLDVAVGSCHSLVA
NFLDGFLKFQTGSNSAFDVV
EVEEPAGPAVLTIGLGHKGR
LAVVLDYTRLNAALGSAAYV
VEDSGCSSSEEVSFQGVGSG
ATLVVTTLGESPTAVSA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 550 |
| 15N chemical shifts | 131 |
| 1H chemical shifts | 825 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TgMIC1-CT | 1 |
Entities:
Entity 1, TgMIC1-CT 137 residues - 14020 Da.
| 1 | LYS | THR | GLU | ILE | HIS | GLY | ASP | SER | THR | LYS | ||||
| 2 | ALA | THR | LEU | GLU | GLU | GLY | GLN | GLN | LEU | THR | ||||
| 3 | LEU | THR | PHE | ILE | SER | THR | LYS | LEU | ASP | VAL | ||||
| 4 | ALA | VAL | GLY | SER | CYS | HIS | SER | LEU | VAL | ALA | ||||
| 5 | ASN | PHE | LEU | ASP | GLY | PHE | LEU | LYS | PHE | GLN | ||||
| 6 | THR | GLY | SER | ASN | SER | ALA | PHE | ASP | VAL | VAL | ||||
| 7 | GLU | VAL | GLU | GLU | PRO | ALA | GLY | PRO | ALA | VAL | ||||
| 8 | LEU | THR | ILE | GLY | LEU | GLY | HIS | LYS | GLY | ARG | ||||
| 9 | LEU | ALA | VAL | VAL | LEU | ASP | TYR | THR | ARG | LEU | ||||
| 10 | ASN | ALA | ALA | LEU | GLY | SER | ALA | ALA | TYR | VAL | ||||
| 11 | VAL | GLU | ASP | SER | GLY | CYS | SER | SER | SER | GLU | ||||
| 12 | GLU | VAL | SER | PHE | GLN | GLY | VAL | GLY | SER | GLY | ||||
| 13 | ALA | THR | LEU | VAL | VAL | THR | THR | LEU | GLY | GLU | ||||
| 14 | SER | PRO | THR | ALA | VAL | SER | ALA |
Samples:
sample_1: TgMIC1-CT, [U-95% 13C; U-95% 15N], 0.5 mM; Sodium Chloride 150 mM; sodium phosphate 20 mM
conditions_1: pH: 7.0; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| HN(CA)CO | not available | not available | not available |
| HNCO | not available | not available | not available |
| HBHA(CBCACO)NH | not available | not available | not available |
| (H)CC(CO)NH TOCSY | not available | not available | not available |
| HCCH TOCSY | not available | not available | not available |
Software:
NMRView v4.1.3 -
XWIN-NMR -
NMRPipe -
NMR spectrometers:
- Bruker DRX 500 MHz
Related Database Links:
| BMRB | 15685 15692 |
| PDB | |
| EMBL | CAA96466 |
| GB | EEB01391 EEE22493 EEE31467 |
| REF | XP_002368531 |
| SP | O00834 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts