BMRB Entry 6382
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6382
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Title: 1H, 13C, and 15N Chemical Shift Assignments for Xenopus prion protein.
Deposition date: 2004-11-10 Original release date: 2005-04-08
Authors: Perez, Daniel; Wuthrich, Kurt
Citation: Perez, Daniel; Wuthrich, Kurt. "NMR assignment of the Xenopus leavis prion protein fragment xlPrP(98-226)" J. Biomol. NMR 31, 260-260 (2005).
Assembly members:
prion, polymer, 130 residues, Formula weight is not available
Natural source: Common Name: African clawed frog Taxonomy ID: 8355 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Xenopus leavis
Experimental source: Production method: recombinant technology Host organism: Eschericia coli
Entity Sequences (FASTA):
prion: GSGYNKQWKPPKSKTNMKSV
AIGAAAGAIGGYMLGNAVGR
MSYQFNNPMESRYYNDYYNQ
MPNRVYRPMYRGEEYVSEDR
FVRDCYNMSVTEYIIKPAEG
KNNSELNQLDTTVKSQIIRE
MCITEYRRGS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 416 |
| 15N chemical shifts | 150 |
| 1H chemical shifts | 908 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | prion protein | 1 |
Entities:
Entity 1, prion protein 130 residues - Formula weight is not available
| 1 | GLY | SER | GLY | TYR | ASN | LYS | GLN | TRP | LYS | PRO | |
| 2 | PRO | LYS | SER | LYS | THR | ASN | MET | LYS | SER | VAL | |
| 3 | ALA | ILE | GLY | ALA | ALA | ALA | GLY | ALA | ILE | GLY | |
| 4 | GLY | TYR | MET | LEU | GLY | ASN | ALA | VAL | GLY | ARG | |
| 5 | MET | SER | TYR | GLN | PHE | ASN | ASN | PRO | MET | GLU | |
| 6 | SER | ARG | TYR | TYR | ASN | ASP | TYR | TYR | ASN | GLN | |
| 7 | MET | PRO | ASN | ARG | VAL | TYR | ARG | PRO | MET | TYR | |
| 8 | ARG | GLY | GLU | GLU | TYR | VAL | SER | GLU | ASP | ARG | |
| 9 | PHE | VAL | ARG | ASP | CYS | TYR | ASN | MET | SER | VAL | |
| 10 | THR | GLU | TYR | ILE | ILE | LYS | PRO | ALA | GLU | GLY | |
| 11 | LYS | ASN | ASN | SER | GLU | LEU | ASN | GLN | LEU | ASP | |
| 12 | THR | THR | VAL | LYS | SER | GLN | ILE | ILE | ARG | GLU | |
| 13 | MET | CYS | ILE | THR | GLU | TYR | ARG | ARG | GLY | SER |
Samples:
sample_1: prion, [U-13C; U-15N], 1 mM
conditions_1: pH: 4.5; temperature: 293.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCA | sample_1 | not available | conditions_1 |
| HNCACB | sample_1 | not available | conditions_1 |
| CBCACONH | sample_1 | not available | conditions_1 |
| HCcH TOCSY | sample_1 | not available | conditions_1 |
| HCcH COSY | sample_1 | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Bruker DRX 500 MHz
- Bruker DRX 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts